Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-5-2F8] to PTPN12
- Suitable for: WB, IP, ICC, IHC-P
- Reacts with: Human
Product nameAnti-PTPN12 antibody [BL-5-2F8]
See all PTPN12 primary antibodies
DescriptionRabbit monoclonal [BL-5-2F8] to PTPN12
Tested applicationsSuitable for: WB, IP, ICC, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PTPN12 aa 499-780. The exact sequence is proprietary. NP_002826.2 and Gene ID 5782.
Database link: Q05209
This product is sold under License from Bethyl Laboratories, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20µl/0.5mg lysate.
|ICC||1/20 - 1/200.|
|IHC-P||1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
- Information by UniProt
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
Formaldehyde-fixed SW620 cells labeling PTPN12 using ab243864 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Formalin-fixed, paraffin-embedded human non-small cell lung cancer tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human Mammary gland tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
PTPN12 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243864 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243864 at 1/1000 dilution.
Lane 1: ab243864 IP in HeLa cell lysate.
Lane 2: Contol IgG in HeLa cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot analysis using ab243864 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (5 µg).
Lane 2: HEK-293T whole cell lysate (5 µg).
Lane3: Jurkat whole cell lysate (5 µg).
Lane 4: MCF-7 whole cell lysate (5 µg).
Lane 5: HepG2 whole cell lysate (5 µg).
Lane 6: A549 whole cell lysate (5 µg).
Lane 7: SW620 whole cell lysate (5 µg).
Lane 8: SK whole cell lysate (5 µg).
Lane 9: HCT 116 whole cell lysate (5 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243864 has not yet been referenced specifically in any publications.