Product nameAnti-PTPN12 antibody [BL-5-2F8]
See all PTPN12 primary antibodies
DescriptionRabbit monoclonal [BL-5-2F8] to PTPN12
Tested applicationsSuitable for: WB, IP, ICC, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PTPN12 aa 499-780. The exact sequence is proprietary. NP_002826.2 and Gene ID 5782.
Database link: Q05209
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20µl/0.5mg lysate.
|ICC||1/20 - 1/200.|
|IHC-P||1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
- Information by UniProt
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
Formaldehyde-fixed SW620 cells labeling PTPN12 using ab243864 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Formalin-fixed, paraffin-embedded human non-small cell lung cancer tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human Mammary gland tissue stained for PTPN12 using ab243864 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
PTPN12 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243864 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243864 at 1/1000 dilution.
Lane 1: ab243864 IP in HeLa cell lysate.
Lane 2: Contol IgG in HeLa cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot analysis using ab243864 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (5 µg).
Lane 2: HEK-293T whole cell lysate (5 µg).
Lane3: Jurkat whole cell lysate (5 µg).
Lane 4: MCF-7 whole cell lysate (5 µg).
Lane 5: HepG2 whole cell lysate (5 µg).
Lane 6: A549 whole cell lysate (5 µg).
Lane 7: SW620 whole cell lysate (5 µg).
Lane 8: SK whole cell lysate (5 µg).
Lane 9: HCT 116 whole cell lysate (5 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
ab243864 has not yet been referenced specifically in any publications.