Anti-PTPN12 antibody - N-terminal (ab154892)
Key features and details
- Rabbit polyclonal to PTPN12 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PTPN12 antibody - N-terminal
See all PTPN12 primary antibodies -
Description
Rabbit polyclonal to PTPN12 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to a region within N terminal amino acids 1-207 of PTPN12 (Q05209)
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Positive control
- Recombinant Human PTPN12 protein (ab132054) can be used as a positive control in WB. A549, HeLa or HepG2 lysate, HeLa cells, D54 xenograft, RT2 xenograft or Human ulcerative colitis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 531877 Cow
- Entrez Gene: 5782 Human
- Entrez Gene: 19248 Mouse
- Entrez Gene: 117255 Rat
- Omim: 600079 Human
- SwissProt: Q05209 Human
- SwissProt: P35831 Mouse
- SwissProt: Q63745 Rat
see all -
Alternative names
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
see all
Images
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Anti-PTPN12 antibody - N-terminal (ab154892) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 88 kDa
5% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human D54 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution.
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Immunohistochemical analysis of paraffin embedded Human RT2 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution.
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Immunohistochemical analysis of paraffin embedded Human ulcerative colitis tissue labeling PTPN12 with ab154892 at a 1/100 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PTPN12 with ab154892 at a 1/200 dilution (top image), and costained with Hoescht 33342 (bottom image)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154892 has been referenced in 1 publication.
- Cheng Y & Liu W MicroRNA-503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12. Exp Ther Med 18:2285-2292 (2019). PubMed: 31410179