Anti-PTPN12 antibody - N-terminal (ab154892)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PTPN12 antibody - N-terminal
See all PTPN12 primary antibodies -
Description
Rabbit polyclonal to PTPN12 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to a region within N terminal amino acids 1-207 of PTPN12 (Q05209)
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Positive control
- Recombinant Human PTPN12 protein (ab132054) can be used as a positive control in WB. A549, HeLa or HepG2 lysate, HeLa cells, D54 xenograft, RT2 xenograft or Human ulcerative colitis tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 88 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 531877 Cow
- Entrez Gene: 5782 Human
- Entrez Gene: 19248 Mouse
- Entrez Gene: 117255 Rat
- Omim: 600079 Human
- SwissProt: Q05209 Human
- SwissProt: P35831 Mouse
- SwissProt: Q63745 Rat
see all -
Alternative names
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
see all
Images
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Western blot - Anti-PTPN12 antibody - N-terminal (ab154892)Anti-PTPN12 antibody - N-terminal (ab154892) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 88 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human D54 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human RT2 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human ulcerative colitis tissue labeling PTPN12 with ab154892 at a 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PTPN12 antibody - N-terminal (ab154892)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PTPN12 with ab154892 at a 1/200 dilution (top image), and costained with Hoescht 33342 (bottom image)
Protocols
References
ab154892 has not yet been referenced specifically in any publications.
