Anti-PTPN12 antibody - N-terminal (ab154892)
Key features and details
- Rabbit polyclonal to PTPN12 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTPN12 antibody - N-terminal
See all PTPN12 primary antibodies -
Description
Rabbit polyclonal to PTPN12 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to a region within N terminal amino acids 1-207 of PTPN12 (Q05209)
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Positive control
- Recombinant Human PTPN12 protein (ab132054) can be used as a positive control in WB. A549, HeLa or HepG2 lysate, HeLa cells, D54 xenograft, RT2 xenograft or Human ulcerative colitis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
Cow
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 531877 Cow
- Entrez Gene: 5782 Human
- Entrez Gene: 19248 Mouse
- Entrez Gene: 117255 Rat
- Omim: 600079 Human
- SwissProt: Q05209 Human
- SwissProt: P35831 Mouse
- SwissProt: Q63745 Rat
see all -
Alternative names
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
see all
Images
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Anti-PTPN12 antibody - N-terminal (ab154892) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 88 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human D54 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human RT2 xenograft tissue labeling PTPN12 with ab154892 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody - N-terminal (ab154892)Immunohistochemical analysis of paraffin embedded Human ulcerative colitis tissue labeling PTPN12 with ab154892 at a 1/100 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PTPN12 with ab154892 at a 1/200 dilution (top image), and costained with Hoescht 33342 (bottom image)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154892 has been referenced in 1 publication.
- Cheng Y & Liu W MicroRNA-503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12. Exp Ther Med 18:2285-2292 (2019). PubMed: 31410179