Overview

  • Product name

  • Description

    Rabbit polyclonal to PTPN14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PTPN14 aa 896-1169.
    Database link: Q15678

  • Positive control

    • Lung papillory adenocarcinoma tissue; A549, HeLa, HepG2 and HCT116 cell lysates.
  • General notes

     This product was previously labelled as PTPD2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/3000. Predicted molecular weight: 135 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using Tris-EDTA buffer ( PH8.0) also possible.

Target

  • Function

    Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis.
  • Tissue specificity

    Expressed in a variety of human tissues including kidney, skeletal muscle, lung and placenta.
  • Involvement in disease

    Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY) [MIM:613611]. A disease characterized by posterior choanal atresia and lymphedema. Additional features are a high-arched palate, hypoplastic nipples, and mild pectus excavatum. Note=A homozygous deletion in PTPN14 predicted to result in frameshift and premature truncation, has been shown to be the cause of choanal atresia and lymphedema in one family.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
    Contains 1 FERM domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytoskeletal associated protein tyrosine phosphatase antibody
    • MGC126803 antibody
    • PEZ antibody
    • Phosphatase with ezrin domain antibody
    • Protein tyrosine phosphatase non receptor type 14 antibody
    • Protein tyrosine phosphatase pez antibody
    • Protein-tyrosine phosphatase pez antibody
    • PTN14_HUMAN antibody
    • PTP 36 antibody
    • PTP36 antibody
    • PTPD 2 antibody
    • PTPN 14 antibody
    • PTPN14 antibody
    • Tyrosine protein phosphatase non receptor type 14 antibody
    • Tyrosine-protein phosphatase non-receptor type 14 antibody
    see all

Images

  • All lanes : Anti-PTPN14 antibody (ab126114) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HCT116 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 135 kDa



    5% SDS Page
  • ab126114, at a 1/500 dilution, staining PTPN14 in paraffin-embedded Human lung papillory adenocarcinoma by immunohistochemistry.

References

ab126114 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse lymphatic endothelial cell)
Permeabilization
Yes - 0.1% TritonX100 in PBS
Specification
mouse lymphatic endothelial cell
Blocking step
1% BSA and 0.3% TritonX100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Aug 29 2016

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