Anti-PTPN18 antibody (ab230300)
Key features and details
- Rabbit polyclonal to PTPN18
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PTPN18 antibody -
Description
Rabbit polyclonal to PTPN18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PTPN18 aa 1-210.
Database link: Q99952 -
Positive control
- WB: Rat heart tissue lysate. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50, 38 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50, 38 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues. -
Tissue specificity
Expressed in brain, colon and several tumor-derived cell lines. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 26469 Human
- Entrez Gene: 301333 Rat
- Omim: 606587 Human
- SwissProt: Q99952 Human
- Unigene: 516390 Human
- Unigene: 726000 Human
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Alternative names
- BDP 1 antibody
- Brain derived phosphatase antibody
- Brain-derived phosphatase antibody
see all
Images
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Anti-PTPN18 antibody (ab230300) at 1/1000 dilution + Rat heart tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 50, 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human brain tissue stained for PTPN18 with ab230300 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab230300 has not yet been referenced specifically in any publications.