Key features and details
- Mouse monoclonal [5H5D7] to PTPRD
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PTPRD antibody [5H5D7]
DescriptionMouse monoclonal [5H5D7] to PTPRD
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PTPRD aa 1077-1265 (extracellular). Expressed in E.Coli.
Database link: P23468
- WB: Human PTPRD (AA: extra 1077-1265) recombinant protein; PTPRD (AA: extra 1077-1265)-hIgGFc transfected HEK-293 cell lysate. Flow: HL-60 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|Flow Cyt||1/200 - 1/400.|
RelevancePTPRD (protein tyrosine phosphatase receptor type D) is a protein tyrosine phosphatase that falls into the receptor-type group as it characteristically comprises an extracellular region, a single transmembrane region and two tandem cytoplasmic catalytic domains. The extracellular region of this protein contains 3 Ig-like and 8 fibronectin type III-like domains. This protein is thought to be involved in promoting neurite growth and in axonal guidance.
Cellular localizationCell Membrane
- HPTP antibody
- HPTP D antibody
- HPTP DELTA antibody
Anti-PTPRD antibody [5H5D7] (ab233806) at 1/500 dilution + Human PTPRD (AA: extra 1077-1265) recombinant protein.
Expected MW is 47.4 kDa.
All lanes : Anti-PTPRD antibody [5H5D7] (ab233806) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : PTPRD (AA: extra 1077-1265)-hIgGFc transfected HEK-293 cell lysate.
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling PTPRD using ab233806 at 1/200 dilution (green) and a negative control (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233806 has been referenced in 1 publication.
- Wu A et al. miR-516a-3p promotes proliferation, migration, and invasion and inhibits apoptosis in lung adenocarcinoma by targeting PTPRD. Int J Clin Exp Pathol 12:4222-4231 (2019). PubMed: 31933822