Product nameAnti-PTPRE antibody [EPR6715]
See all PTPRE primary antibodies
DescriptionRabbit monoclonal [EPR6715] to PTPRE
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PTPRE aa 650-750.
Database link: P23469
- HL60 treated with TPA, HL60, HeLa and C2C12 cell lysates; Permeabilized HL60 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 71, 75, 81 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIsoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.
Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake.
Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
Tissue specificityExpressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells).
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity.
modificationsA catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.
Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.
Isoform 1 is glycosylated.
Cellular localizationCytoplasm; Cell membrane and Cytoplasm. Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane.
- Information by UniProt
- DKFZp313F1310 antibody
- FLJ57799 antibody
- FLJ58245 antibody
All lanes : Anti-PTPRE antibody [EPR6715] (ab126788) at 1/1000 dilution
Lane 1 : HL60 cell lysate, treated with TPA
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71, 75, 81 kDa
ab126788 at 1/10 dilution staining PTPRE in permeabilized HL60 cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126788 has not yet been referenced specifically in any publications.