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    ptprg-antibody-ab12555.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases
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Anti-PTPRG antibody (ab12555)

  • Datasheet
  • SDS
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Western blot - Anti-PTPRG antibody (ab12555)

    Key features and details

    • Rabbit polyclonal to PTPRG
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant human PTPRG protein (ab42587)

    View more associated products

    Overview

    • Product name

      Anti-PTPRG antibody
    • Description

      Rabbit polyclonal to PTPRG
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human PTPRG aa 1000-1100 (internal sequence) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab12588)

    • Positive control

      • This antibody gave a positive signal with PTPRG recombinant domain
    • General notes

       This product was previously labelled as PTP gamma

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Phosphatases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human PTPRG protein (ab42587)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab12555 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    All applications
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 50-70 kDa (predicted molecular weight: 50-70 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 50-70 kDa (predicted molecular weight: 50-70 kDa).

    Target

    • Function

      Possesses tyrosine phosphatase activity.
    • Tissue specificity

      Found in a variety of tissues.
    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.
      Contains 1 alpha-carbonic anhydrase domain.
      Contains 1 fibronectin type-III domain.
      Contains 2 tyrosine-protein phosphatase domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P23470 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5793 Human
      • Omim: 176886 Human
      • SwissProt: P23470 Human
      • Unigene: 595541 Human
      • Alternative names

        • H RG317H01.1 antibody
        • HPTPG antibody
        • Protein tyrosine phosphatase gamma antibody
        • Protein tyrosine phosphatase receptor type G antibody
        • Protein tyrosine phosphatase receptor type gamma polypeptide antibody
        • Protein-tyrosine phosphatase gamma antibody
        • PTPG antibody
        • PTPgamma antibody
        • Ptprg antibody
        • PTPRG protein antibody
        • PTPRG_HUMAN antibody
        • R PTP gamma antibody
        • R-PTP-gamma antibody
        • Receptor type protein phosphatase gamma antibody
        • Receptor type tyrosine protein phosphatase gamma antibody
        • Receptor tyrosine phosphatase gamma antibody
        • Receptor-type tyrosine-protein phosphatase gamma antibody
        • RPTPG antibody
        see all

      Images

      • Western blot - Anti-PTPRG antibody (ab12555)
        Western blot - Anti-PTPRG antibody (ab12555)
        All lanes : Anti-PTPRG antibody (ab12555) at 1 µg/ml

        Lane 1 : 0.1µg GLEPP1 (PTP RO)
        Lane 2 : 0.1µg SAP1 (PTP RH)
        Lane 3 : 0.1µg PTPRG
        Lane 4 : 0.1µg PTP beta (PTP B)
        Lane 5 : 0.1µg DEP1 (PTP RJ)
        Lane 6 : 0.1µg PTP kappa (PTP RK)
        Lane 7 : 0.1µg PCPTP1 (PTP RR)
        Lane 8 : 0.1µg LAR (PTP RF)

        Performed under reducing conditions.

        Predicted band size: 50-70 kDa
        Observed band size: 50-70 kDa



        Recombinant PTP phosphatase domains (0.1µg) were probed with ab12555 at 1/1000.  The antibody recognises the recombinant PTPRG fusion protein at 50-70kDa.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab12555? Please let us know so that we can cite the reference in this datasheet.

      ab12555 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I want to buy ptprg antibody of your company.The catalog shows two kinds of ptprg antibody.their molecular weight are different,so I want to know the exact molecular weight of ptprg and the difference of ab12555 and ab37525 you applied.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 24 2006

      Answer

      Thank you for your enquiry. The product ab12555 recognizes a PTP gamma fusion protein of approximately 50-70 kDa. The product ab37525 recognizes the unprocessed precursor protein which has a molecular weight of 162 kDa. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Dec 26 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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