Key features and details
- Rabbit polyclonal to PTPRH
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-PTPRH antibody
See all PTPRH primary antibodies
DescriptionRabbit polyclonal to PTPRH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment corresponding to Human PTPRH aa 844-1096. Expressed in E.coli.
NNAKNRYRNVLPYDWSRVPLKPIHEEPGSDYINASFMPGLWSPQEFIATQ GPLPQTVGDFWRLVWEQQSHTLVMLTNCMEAGRVKCEHYWPLDSQPCTHG HLRVTLVGEEVMENWTVRELLLLQVEEQKTLSVRQFHYQAWPDHGVPSSP DTLLAFWRMLRQWLDQTMEGGPPIVHCSAGVGRTGTLIALDVLLRQLQSE GLLGPFSFVRKMRESRPLMVQTEAQYVFLHQCILRFLQQSAQAPAEKEVP YED
Database link: Q9HD43
- WB: Recombinant human PTPRH protein; HeLa cell lysate; pig brain lysate. IHC-P: Human liver, skin cancer and stomach cancer tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.05% Proclin 300, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 122 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion-associated substrates and thus negatively regulating integrin-promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms: inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of p130cas, focal adhesion kinase and c-Src. Reduces migratory activity of Jurkat cells.
Tissue specificityExpressed at high levels in the brain, spleen and liver and at lower levels in the heart and stomach. Expressed in pancreatic and colorectal cancer cells, but not in normal pancreas or colon. Expression in hepatocellular carcinoma is related to the differentiation status of the tumor and expression is inversely related to tumor aggressiveness.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
Contains 8 fibronectin type-III domains.
Contains 1 tyrosine-protein phosphatase domain.
DomainThe extracellular domain mediates homodimerization. One or more cysteines in the extracellular domain is essential for the formation of dimers probably by forming a disulfide bond.
The cytoplasmic domain mediates the interaction with LCK.
Cellular localizationMembrane. Cytoplasm.
- Information by UniProt
- BEM 2 antibody
- BEM2 antibody
- Brain-enriched membrane-associated protein tyrosine phosphatase 2 antibody
All lanes : Anti-PTPRH antibody (ab231723) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig brain lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 122 kDa
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for PTPRH using ab231723 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for PTPRH using ab231723 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for PTPRH using ab231723 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Anti-PTPRH antibody (ab231723) at 3 µg/ml + Recombinant human PTPRH protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 122 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231723 has not yet been referenced specifically in any publications.