Product nameAnti-PTPRK antibody
See all PTPRK primary antibodies
DescriptionRabbit polyclonal to PTPRK
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Human
Synthetic peptide corresponding to Human PTPRK aa 900-1000 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive result in PTP beta (PTPRB) and PTPRK recombinant protein domains
This product was previously labelled as PTP kappa
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab13225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).|
FunctionRegulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa.
Tissue specificityHigh levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 MAM domain.
Contains 2 tyrosine-protein phosphatase domains.
modificationsThis protein undergoes proteolytic processing.
Cellular localizationCell junction > adherens junction. Cell membrane.
- Information by UniProt
- dJ480J14.2.1 antibody
- DKFZp686C2268 antibody
- DKFZp779N1045 antibody
All lanes : Anti-PTPRK antibody (ab13225) at 1 µg/ml
Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
Lane 2 : 0.1µg SAP 1 (PTPRH) protein
Lane 3 : 0.1µg PTP gamma (PTPRG) protein
Lane 4 : 0.1µg PTP beta (PTPRB) protein
Lane 5 : 0.1µg DEP1 (PTPRJ) protein
Lane 6 : 0.1µg PTP kappa (PTPRK) protein
Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
Lane 8 : 0.1µg LAR (PTPRF) protein
Performed under reducing conditions.
Predicted band size: 50-70 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Recombinant PTP phosphatase domains (0.1µg of protein) were probed with ab13225 at 1/1000. The antibody recognises the recombinant PTPRK fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.
Lane # Recombinant Protein
1 GLEPP1 (PTP RO)
2 SAP 1 (PTP RH)
3 PTP gamma (PTP RG)
4 PTP beta (PTP B)
This product has been referenced in:
- Agarwal S et al. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PLoS One 8:e62852 (2013). WB ; Human . Read more (PubMed: 23696788) »
- Flavell JR et al. Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells. Blood 111:292-301 (2008). Read more (PubMed: 17720884) »