Anti-PTPRK antibody (ab13225)

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Western blot - Anti-PTPRK antibody (ab13225)

    Key features and details

    • Rabbit polyclonal to PTPRK
    • Suitable for: WB
    • Reacts with: Human, Recombinant fragment
    • Isotype: IgG

    Overview

    • Product name

      Anti-PTPRK antibody
      See all PTPRK primary antibodies

    • Description

      Rabbit polyclonal to PTPRK

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human PTPRK aa 900-1000 (internal sequence) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab13232)

    • Positive control

      • This antibody gave a positive result in PTP beta (PTPRB) and PTPRK recombinant protein domains

    • General notes

       This product was previously labelled as PTP kappa

       

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab13225 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    All applications
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).

    Target

    • Function

      Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa.

    • Tissue specificity

      High levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma.

    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 MAM domain.
      Contains 2 tyrosine-protein phosphatase domains.

    • Post-translational
      modifications

      This protein undergoes proteolytic processing.

    • Cellular localization

      Cell junction > adherens junction. Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • dJ480J14.2.1 antibody
        • DKFZp686C2268 antibody
        • DKFZp779N1045 antibody
        • OTTHUMP00000017180 antibody
        • OTTHUMP00000017181 antibody
        • OTTHUMP00000017182 antibody
        • OTTHUMP00000017187 antibody
        • OTTHUMP00000040306 antibody
        • Protein tyrosine phosphatase kappa antibody
        • Protein tyrosine phosphatase kappa precursor antibody
        • Protein tyrosine phosphatase receptor type K antibody
        • Protein tyrosine phosphatase receptor type kappa antibody
        • Protein-tyrosine phosphatase kappa antibody
        • PTPK antibody
        • PTPkappa antibody
        • Ptprk antibody
        • PTPRK_HUMAN antibody
        • R PTP kappa antibody
        • R-PTP-kappa antibody
        • Receptor type tyrosine protein phosphatase kappa antibody
        • Receptor-type tyrosine-protein phosphatase kappa antibody
        see all

      Images

      • Western blot - Anti-PTPRK antibody (ab13225)
        Western blot - Anti-PTPRK antibody (ab13225)
        All lanes : Anti-PTPRK antibody (ab13225) at 1 µg/ml

        Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
        Lane 2 : 0.1µg SAP 1 (PTPRH) protein
        Lane 3 : 0.1µg PTP gamma (PTPRG) protein
        Lane 4 : 0.1µg PTP beta (PTPRB) protein
        Lane 5 : 0.1µg DEP1 (PTPRJ) protein
        Lane 6 : 0.1µg PTP kappa (PTPRK) protein
        Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
        Lane 8 : 0.1µg LAR (PTPRF) protein

        Performed under reducing conditions.

        Predicted band size: 50-70 kDa
        Observed band size: 65 kDa
        why is the actual band size different from the predicted?



        Recombinant PTP phosphatase domains (0.1µg of protein) were probed with ab13225 at 1/1000.  The antibody recognises the recombinant PTPRK fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.

        Lane #         Recombinant Protein

        1                  GLEPP1 (PTP RO)
        2                  SAP 1 (PTP RH)
        3                  PTP gamma (PTP RG)
        4                  PTP beta (PTP B)
        5        &nbsp

      References (2)

      Publishing research using ab13225? Please let us know so that we can cite the reference in this datasheet.

      ab13225 has been referenced in 2 publications.

      • Agarwal S  et al. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PLoS One 8:e62852 (2013). WB ; Human . PubMed: 23696788
      • Flavell JR  et al. Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells. Blood 111:292-301 (2008). PubMed: 17720884

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Customer contacted us to inquire about the WB image and expected band size when using this product.

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      Answer

      Thank you for contacting us.

      I have been informed that this product is tested using recombinant PTP phosphatase domains. The expected size for these recombinant peptides is around ˜65kDa. We have not tested this in endogenous systems however it is expected that PTPk targets will be around 160 kDa.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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      Question

      Inquiry: I am looking for an antibody that will work on Human PTPRK in an immunoblot and with FACS as well. Can you tell me if ab13225 will do the job? Does it bind inter or extracellularly?

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      Answer

      Thank you for your enquiry and your interest.

      This is a polyclonal antibody so it binds to different epitopes. Thus for polyclonal antibodies epitope mapping is usually not carried out.

      I can confirm that ab13225 has only been tested on Western blot application. Currently, we do not have any information if ab13225 recognizes the native form of the protein or only the denatured form.

      I could offer you a testing discount if you wish to test it in Flow cytometry/FACS. I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/abtrial.

      If you need any further assistance in the future, please do not hesitate to contact me.

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