Anti-PTPRN2 antibody (ab244293)
Key features and details
- Rabbit polyclonal to PTPRN2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PTPRN2 antibody
See all PTPRN2 primary antibodies -
Description
Rabbit polyclonal to PTPRN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PTPRN2 aa 350-500.
Database link: Q92932 -
Positive control
- IHC-P: Human liver tissue. WB: U-87 MG cell lysate. ICC/IF: AF22 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 111 kDa.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 111 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Implicated in development of nervous system and pancreatic endocrine cells. -
Tissue specificity
Highest levels in brain and pancreas. Lower levels in trachea, prostate, stomach and spinal chord. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Domain
The cytoplasmic domain appears to contain the autoantigenic epitopes. -
Post-translational
modificationsAppears to undergo multiple proteolytic cleavage at consecutive basic residues. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 5799 Human
- Omim: 601698 Human
- SwissProt: Q92932 Human
- Unigene: 490789 Human
-
Alternative names
- IA 2beta antibody
- IA2beta antibody
- IAR antibody
see all
Images
-
PFA fixed, Triton X-100 permeabilized AF22 cells labeling PTPRN2 using ab244293 at 4 µg/ml (green) in ICC/IF.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for PTPRN2 with ab244293 at a 1/50 dilution in immunohistochemical analysis.
-
Anti-PTPRN2 antibody (ab244293) at 0.4 µg/ml + U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Predicted band size: 111 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab244293 has not yet been referenced specifically in any publications.