Key features and details
- Rabbit polyclonal to PTPRN2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTPRN2 antibody
See all PTPRN2 primary antibodies
DescriptionRabbit polyclonal to PTPRN2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human liver tissue. WB: U-87 MG cell lysate. ICC/IF: AF22 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 111 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionImplicated in development of nervous system and pancreatic endocrine cells.
Tissue specificityHighest levels in brain and pancreas. Lower levels in trachea, prostate, stomach and spinal chord.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
DomainThe cytoplasmic domain appears to contain the autoantigenic epitopes.
modificationsAppears to undergo multiple proteolytic cleavage at consecutive basic residues.
- Information by UniProt
- IA 2beta antibody
- IA2beta antibody
- IAR antibody
PFA fixed, Triton X-100 permeabilized AF22 cells labeling PTPRN2 using ab244293 at 4 µg/ml (green) in ICC/IF.
Formalin-fixed, paraffin-embedded human liver tissue stained for PTPRN2 with ab244293 at a 1/50 dilution in immunohistochemical analysis.
Anti-PTPRN2 antibody (ab244293) at 0.4 µg/ml + U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Predicted band size: 111 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244293 has not yet been referenced specifically in any publications.