Overview

  • Product name

    Anti-PTPRT antibody [AFT20]
    See all PTPRT primary antibodies
  • Description

    Mouse monoclonal [AFT20] to PTPRT
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PTPRT.

  • Positive control

    • Bosc23 cell transfected with Myc/His/R PTPRT.
  • General notes

     This product was previously labelled as PTP rho

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: 0.87% Sodium chloride, HEPES, 0.01% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Monoclonal
  • Clone number

    AFT20
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 164 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function

    May be involved in both signal transduction and cellular adhesion in the CNS.
  • Tissue specificity

    Expressed in colon, lung, heart and testis, as well as in fetal and adult brain. Not detected in muscle and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 MAM domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein tyrosine phosphatase receptor type T antibody
    • PTPRT antibody
    • PTPRT_HUMAN antibody
    • R PTP T antibody
    • R-PTP-T antibody
    • Receptor protein tyrosine phosphatase antibody
    • Receptor-type tyrosine-protein phosphatase rho antibody
    • Receptor-type tyrosine-protein phosphatase T antibody
    • RPTP-rho antibody
    • RPTPmam4 antibody
    • RPTPrho antibody
    see all

Images

  • All lanes : Anti-PTPRT antibody [AFT20] (ab75521) at 1/10000 dilution

    Lane 1 : Bosc23 Cell Lysate
    Lane 2 : Bosc23 Cell Lysate Transfected with Myc/MKP2
    Lane 3 : Bosc23 Cell Lysate Transfected with Myc/R-PTPRT
    Lane 4 : Bosc23 Cell Lysate Transfected with GFP/Ub

    Predicted band size: 164 kDa

  • All lanes : Anti-PTPRT antibody [AFT20] (ab75521) at 1/2000 dilution

    Lane 1 : Bosc23 cell lysate
    Lane 2 : Bosc23 cell lysate transfected with Myc/His/R PTPRT (887 - 1169 aa)

    Predicted band size: 164 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

References

ab75521 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75521.
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