Key features and details
- Rabbit polyclonal to PTRF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTRF antibody
See all PTRF primary antibodies
DescriptionRabbit polyclonal to PTRF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Opossum, Bat, Elephant
Synthetic peptide corresponding to a region between residue 125 and 175 of human PTRF (NP_036364.2)
- HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab76919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 43 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
Involvement in diseaseCongenital generalized lipodystrophy 4
Sequence similaritiesBelongs to the PTRF/SDPR family.
modificationsPhosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
Cellular localizationMembrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
- Information by UniProt
- 2310075E07Rik antibody
- AW546441 antibody
- Cavin 1 antibody
All lanes : Anti-PTRF antibody (ab76919) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 43 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Western Blot of Immunprecipitate.
ab76919 at 1µg/ml (for WB) staining PTRF in HeLa whole cell lysate,
immunoprecipitated using ab76919 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab76919 has not yet been referenced specifically in any publications.