Product nameAnti-PTRF antibody
See all PTRF primary antibodies
DescriptionRabbit polyclonal to PTRF
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse PTRF aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following mouse whole cell lysates: NIH3T3; MEF1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 44 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionPlays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
Involvement in diseaseCongenital generalized lipodystrophy 4
Sequence similaritiesBelongs to the PTRF/SDPR family.
modificationsPhosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.
Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
Cellular localizationMembrane > caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm > cytosol. Mitochondrion. Nucleus. Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co-localizes with CAV1 in lipid rafts in adipocytes.
- Information by UniProt
- 2310075E07Rik antibody
- AW546441 antibody
- Cavin 1 antibody
All lanes : Anti-PTRF antibody (ab88213) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
PTRF contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 56 kDa band is comparable to molecular weights seen with other commercially available antibodies to PTRF.
ICC/IF image of ab88213 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab88213, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal Goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab88213 has not yet been referenced specifically in any publications.