Overview

  • Product name

  • Description

    Rabbit polyclonal to PTS/PTPS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PTS/PTPS aa 1-111.
    Database link: NP_000308

  • Positive control

    • 293T, H1299 and HeLa cells
  • General notes

     This product was previously labelled as PTS

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.

Target

  • Function

    Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
  • Pathway

    Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
  • Involvement in disease

    Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet.
  • Sequence similarities

    Belongs to the PTPS family.
  • Post-translational
    modifications

    Phosphorylation of Ser-19 is required for maximal enzyme activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6 pyruvoyl tetrahydrobiopterin synthase antibody
    • 6 pyruvoyl tetrahydropterin synthase antibody
    • 6 pyruvoyltetrahydropterin synthase antibody
    • 6-pyruvoyl tetrahydrobiopterin synthase antibody
    • EC 4.2.3.12 antibody
    • FLJ97081 antibody
    • OTTHUMP00000235385 antibody
    • PTP synthase antibody
    • PTPS antibody
    • PTPS_HUMAN antibody
    • PTS antibody
    see all

Images

  • All lanes : Anti-PTS/PTPS antibody (ab96104) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : PTS/PTPS Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 16 kDa

  • Anti-PTS/PTPS antibody (ab96104) at 1/1000 dilution + H1299 whole cell lysate

    at 30 µg

    Predicted band size: 16 kDa



    15% SDS PAGE

References

ab96104 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
0.2 µg
Treatment
hypoxia 40 minutes at E25
Specification
brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 19 2018

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