Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19)
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-PTS/PTPS (phospho S19) antibody [EPR1517(2)]
See all PTS/PTPS primary antibodies
DescriptionRabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19)
ab109462 only detects PTS/PTPS phosphorylated at Serine 19.
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PTS/PTPS.
- HUVEC cell lysates; Human brain tissue; HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PTS
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109462 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
|ICC/IF||1/250 - 1/500.|
FunctionInvolved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
PathwayCofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
Involvement in diseaseDefects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet.
Sequence similaritiesBelongs to the PTPS family.
modificationsPhosphorylation of Ser-19 is required for maximal enzyme activity.
- Information by UniProt
- 6 pyruvoyl tetrahydrobiopterin synthase antibody
- 6 pyruvoyl tetrahydropterin synthase antibody
- 6 pyruvoyltetrahydropterin synthase antibody
All lanes : Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HUVEC cell lysates treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in HeLa cells by Immunofluorescence.
ab109462 has not yet been referenced specifically in any publications.