Recombinant Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)
![Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-122346-anti-pts-phospho-s19-antibody-epr15172-western-blot.jpg "Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)]
See all PTS/PTPS primary antibodies -
Description
Rabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HUVEC cell lysates; Human brain tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1517(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
|
| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
|
ICC/IF
1/250 - 1/500. |
Target
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Function
Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin. -
Pathway
Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3. -
Involvement in disease
Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet. -
Sequence similarities
Belongs to the PTPS family. -
Post-translational
modificationsPhosphorylation of Ser-19 is required for maximal enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 5805 Human
- GenBank: NP_000308.1 Human
- Omim: 612719 Human
- SwissProt: Q03393 Human
- Unigene: 503860 Human
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Alternative names
- 6 pyruvoyl tetrahydrobiopterin synthase antibody
- 6 pyruvoyl tetrahydropterin synthase antibody
- 6 pyruvoyltetrahydropterin synthase antibody
see all
Images
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Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)All lanes : Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HUVEC cell lysates treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-122345-anti-pts-phospho-s19-antibody-epr15172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-122344-anti-pts-phospho-s19-antibody-epr15172-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in HeLa cells by Immunofluorescence.
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![Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-4-benefits-of-recombinant-antibodies.png)
Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109462 has not yet been referenced specifically in any publications.