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    pttg1ip-antibody-ab128040.pdf

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Signal Transduction Protein Trafficking Nuclear Import / Export
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Anti-PTTG1IP antibody (ab128040)

  • Datasheet
Submit a review Submit a question References (1)

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Abpromise

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Find out more.

Western blot - Anti-PTTG1IP antibody (ab128040)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTTG1IP antibody (ab128040)

Key features and details

  • Rabbit polyclonal to PTTG1IP
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PTTG1IP antibody
    See all PTTG1IP primary antibodies
  • Description

    Rabbit polyclonal to PTTG1IP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, corresponging to an internal region of Human PTTG1IP.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab128040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May facilitate PTTG1 nuclear translocation.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 PSI domain.
  • Cellular localization

    Membrane. Cytoplasm. Nucleus. According to PubMed:10781616, it is found in the cytoplasm and the nucleus.
  • Target information above from: UniProt accession P53801 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 754 Human
    • Entrez Gene: 108705 Mouse
    • Entrez Gene: 365548 Rat
    • Omim: 603784 Human
    • SwissProt: P53801 Human
    • SwissProt: Q8R143 Mouse
    • SwissProt: Q6P767 Rat
    • Unigene: 474010 Human
    • Unigene: 28853 Mouse
    • Unigene: 4248 Rat
    see all
  • Alternative names

    • 1810010L20Rik antibody
    • AI314311 antibody
    • AU018448 antibody
    • C21orf1 antibody
    • C21orf3 antibody
    • C79540 antibody
    • chromosome 21 open reading frame 1 antibody
    • MGC36923 antibody
    • PBF antibody
    • Pituitary tumor transforming 1 interacting protein antibody
    • Pituitary tumor transforming gene 1 protein interacting protein antibody
    • Pituitary tumor transforming gene protein binding factor antibody
    • Pituitary tumor-transforming gene 1 protein-interacting protein antibody
    • Pituitary tumor-transforming gene protein-binding factor antibody
    • PTTG binding factor antibody
    • PTTG-binding factor antibody
    • PTTG_HUMAN antibody
    • Pttg1ip antibody
    • Putative surface glycoprotein C21orf1 precursor antibody
    see all

Images

  • Western blot - Anti-PTTG1IP antibody (ab128040)
    Western blot - Anti-PTTG1IP antibody (ab128040)
    All lanes : Anti-PTTG1IP antibody (ab128040) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : RAW264.7 cell extract with synthesized peptide

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTTG1IP antibody (ab128040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTTG1IP antibody (ab128040)
    ab128040, at a concentration of 5 µg/ml, staining PTTG1IP in paraffin-embedded Human Prostate, Epithelium tissue by Immunohistochemistry.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab128040? Please let us know so that we can cite the reference in this datasheet.

    ab128040 has been referenced in 1 publication.

    • Repo H  et al. PTTG1-interacting protein (PTTG1IP/PBF) predicts breast cancer survival. BMC Cancer 17:705 (2017). PubMed: 29078751

    Customer reviews and Q&As

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