Recombinant
RabMAb

Recombinant Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

Overview

  • Product name

    Anti-PU.1/Spi1 antibody [EPR3158Y]
    See all PU.1/Spi1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3158Y] to PU.1/Spi1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PU.1/Spi1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P17947

  • Positive control

    • Daudi and U937 cell lysates. Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000 - 1/20000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Images

    • All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/10000 dilution

      Lane 1 : Daudi cell lysates
      Lane 2 : U937 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa
      Observed band size: 42 kDa
      why is the actual band size different from the predicted?

    • ab76543 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab76543, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab76543 showing positive staining in Normal lung lymphocytes staining tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab76543 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhang K  et al. PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell Death Dis 9:312 (2018). Read more (PubMed: 29472524) »
    • Mohr S  et al. Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia. Cancer Cell 31:549-562.e11 (2017). IHC . Read more (PubMed: 28399410) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse prefrontal cortex)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% BisTris)
    Loading amount
    20 µg
    Specification
    Mouse prefrontal cortex
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Sample
    Human Tissue sections (Lymph node)
    Specification
    Lymph node
    Permeabilization
    No
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Jul 15 2013

    Answer

    DISCOUNT CODE: 42636
    Expiration date:15-Mar-13

    I am very pleased to hear a customer would like to accept our offer and test ab76543 in IHC-Fr. To redeem this offer, an Abreview needs to be submitted for IHC-Fr on human samples and this code put in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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