Recombinant
RabMAb

Anti-PU.1/Spi1 antibody [EPR3159Y] (ab76542)

Overview

  • Product name
    Anti-PU.1/Spi1 antibody [EPR3159Y]
    See all PU.1/Spi1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3159Y] to PU.1/Spi1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PU.1/Spi1 (C terminal). The exact sequence is proprietary.

  • Positive control
    • U937 and TF-1 cell lysates. Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
    • Sequence similarities
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Images

    • All lanes : Anti-PU.1/Spi1 antibody [EPR3159Y] (ab76542) at 1/1000 dilution

      Lane 1 : U937 cell lysates
      Lane 2 : TF-1 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa
      Observed band size: 42 kDa (why is the actual band size different from the predicted?)

    • ab76542, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.

    References

    ab76542 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76542.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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