Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

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Overview

  • Product name

    Anti-PU.1/Spi1 antibody [PU1/2146]
    See all PU.1/Spi1 primary antibodies

  • Description

    Mouse monoclonal [PU1/2146] to PU.1/Spi1

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human PU.1/Spi1 aa 16-170. The exact sequence is proprietary.
    Database link: P17947

  • Positive control

    • IHC-P: Human Hodgkin's lymphoma, colon carcinoma, lymph node and spleen tissues. WB: THP-1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab238054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary ab for 30 minutes at RT
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 31 kDa.

Target

  • Function

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Images

    • Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      ab238054 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054) at 1 µg/ml + THP-1 (human monocytic leukemia cell line) whole cell lysate

      Predicted band size: 31 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    References

    ab238054 has not yet been referenced specifically in any publications.

    Publishing research using ab238054? Please let us know so that we can cite the reference in this datasheet.

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