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  1. Link

    pu1spi1-antibody-pu12146-ab238054.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

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Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
  • Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

Key features and details

  • Mouse monoclonal [PU1/2146] to PU.1/Spi1
  • Suitable for: Protein Array, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-PU.1/Spi1 antibody [PU1/2146]
    See all PU.1/Spi1 primary antibodies
  • Description

    Mouse monoclonal [PU1/2146] to PU.1/Spi1
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PU.1/Spi1 aa 16-170. The exact sequence is proprietary.
    Database link: P17947

  • Positive control

    • IHC-P: Human Hodgkin's lymphoma, colon carcinoma, lymph node and spleen tissues. WB: THP-1 cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Ab purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    PU1/2146
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Ets
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Associated products

  • Alternative Versions

    • Anti-PU.1/Spi1 antibody [PU1/2146] - BSA and Azide free (ab237905)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human PU.1/Spi1 protein (denatured) (ab202185)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary ab for 30 minutes at RT

WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 31 kDa.
Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary ab for 30 minutes at RT

WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 31 kDa.

Target

  • Function

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17947 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6688 Human
    • Omim: 165170 Human
    • SwissProt: P17947 Human
    • Unigene: 502511 Human
    • Alternative names

      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    Images

    • Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      ab238054 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054) at 1 µg/ml + THP-1 (human monocytic leukemia cell line) whole cell lysate

      Predicted band size: 31 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)

      Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab238054? Please let us know so that we can cite the reference in this datasheet.

    ab238054 has not yet been referenced specifically in any publications.

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