Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)
![Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)](https://www.abcam.com/ps/products/238/ab238054/Images/ab238054-336527-anti-pu1-spi1-antibody-pu1-2146-protein-array.jpg "Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)")
Key features and details
- Mouse monoclonal [PU1/2146] to PU.1/Spi1
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PU.1/Spi1 antibody [PU1/2146]
See all PU.1/Spi1 primary antibodies -
Description
Mouse monoclonal [PU1/2146] to PU.1/Spi1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanProtein Array HumanWB Human
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Immunogen
Recombinant fragment within Human PU.1/Spi1 aa 16-170. The exact sequence is proprietary.
Database link: P17947 -
Positive control
- IHC-P: Human Hodgkin's lymphoma, colon carcinoma, lymph node and spleen tissues. WB: THP-1 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Ab purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
PU1/2146 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| Protein Array |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary ab for 30 minutes at RT |
|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 31 kDa.
|
| Notes |
|---|
|
Protein Array
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary ab for 30 minutes at RT |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 31 kDa. |
Target
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Function
Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6688 Human
- Omim: 165170 Human
- SwissProt: P17947 Human
- Unigene: 502511 Human
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Alternative names
- transcription factor spi1 antibody
- 31 kDa Transforming Protein antibody
- 31 kDa-transforming protein antibody
see all
Images
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Protein Array - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)ab238054 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)](https://www.abcam.com/ps/products/238/ab238054/Images/ab238054-315598-anti-pu1-spi1-antibody-pu1-2146-western-blot.jpg)
Western blot - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054) at 1 µg/ml + THP-1 (human monocytic leukemia cell line) whole cell lysate
Predicted band size: 31 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)](https://www.abcam.com/ps/products/238/ab238054/Images/ab238054-315601-anti-pu1-spi1-antibody-pu1-2146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)Formalin-fixed, paraffin-embedded human spleen tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)](https://www.abcam.com/ps/products/238/ab238054/Images/ab238054-315600-anti-pu1-spi1-antibody-pu1-2146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)Formalin-fixed, paraffin-embedded human lymph node tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)](https://www.abcam.com/ps/products/238/ab238054/Images/ab238054-315599-anti-pu1-spi1-antibody-pu1-2146-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)](https://www.abcam.com/ps/products/238/ab238054/Images/ab238054-315595-anti-pu1-spi1-antibody-pu1-2146-microtomy-formalin-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (ab238054)Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for PU.1/Spi1 using ab238054 at 2 μg/mL in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238054 has not yet been referenced specifically in any publications.