Overview

  • Product name

    Anti-PU.1/Spi1 antibody [SP238]
    See all PU.1/Spi1 primary antibodies
  • Description

    Rabbit monoclonal [SP238] to PU.1/Spi1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide within Human PU.1/Spi1 aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: P17947

  • Positive control

    • IHC-P: Human tonsil lysate. FC: THP-1 cells. ICC/IF: Raji cells.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000.
ICC/IF 1/1000.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • transcription factor spi1 antibody
    • 31 kDa Transforming Protein antibody
    • 31 kDa-transforming protein antibody
    • cb1086 antibody
    • Hematopoietic transcription factor PU.1 antibody
    • OF antibody
    • oncogene spi1 antibody
    • PU.1 antibody
    • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
    • SFPI1 antibody
    • si:by184l24.2 antibody
    • SPI 1 antibody
    • SPI 1 proto oncogene antibody
    • SPI A antibody
    • Spi1 antibody
    • SPI1_HUMAN antibody
    • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
    • Spleen focus forming virus proviral integration oncogene spi1 antibody
    • Transcription factor PU.1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of THP-1 (human monocytic leukemia monocyte) cells labeling PU.1/Spi1 with purifiedab183327 at 1:200 dilution (0.715 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PU.1/Spi1 with ab183327 at 1/100.

References

ab183327 has not yet been referenced specifically in any publications.

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