Key features and details
- Rabbit polyclonal to PUF60/FIR
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PUF60/FIR antibody
See all PUF60/FIR primary antibodies
DescriptionRabbit polyclonal to PUF60/FIR
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
Synthetic peptide within Human PUF60/FIR aa 509-559. The exact sequence is proprietary. (NP_510965.1).
Database link: Q9UHX1
- IHC-P: Human prostate carcinoma tissue; mouse teratoma tissue. WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate (ab150035).
This product was previously labelled as PUF60
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225698 was affinity purified using an epitope specific to PUF60/FIR immobilized on solid support.
Our Abpromise guarantee covers the use of ab225698 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionDNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
Tissue specificityIsoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Sequence similaritiesBelongs to the RRM half pint family.
Contains 3 RRM (RNA recognition motif) domains.
DomainThe third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions.
Cellular localizationNucleus. Colocalizes partially with TROVE2.
- Information by UniProt
- 60 kDa poly(U)-binding-splicing factor antibody
- FBP-interacting repressor antibody
- FIR antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for PUF60/FIR using ab225698 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PUF60/FIR using ab225698 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes :
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 minutes
ab225698 has not yet been referenced specifically in any publications.