Our Abpromise guarantee covers the use of ab22819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 58 kDa.
ELISA 1/32000.


  • Function

    DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
  • Tissue specificity

    Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the RRM half pint family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Domain

    The third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions.
  • Cellular localization

    Nucleus. Colocalizes partially with TROVE2.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60 kDa poly(U)-binding-splicing factor antibody
    • FBP-interacting repressor antibody
    • FIR antibody
    • FLJ31379 antibody
    • FUSE-binding protein-interacting repressor antibody
    • Poly U binding splicing factor 60KDa antibody
    • Poly(U) binding splicing factor 60 antibody
    • Poly(U)-binding-splicing factor PUF60 antibody
    • poly-U binding splicing factor PUF60 antibody
    • PUF60 antibody
    • PUF60_HUMAN antibody
    • pyrimidine tract binding splicing factor antibody
    • Ro ribonucleoprotein-binding protein 1 antibody
    • Ro-binding protein 1 antibody
    • RoBP1 antibody
    • RoBPI antibody
    • siah binding protein 1 antibody
    • Siah-binding protein 1 antibody
    • Siah-BP1 antibody
    • SIAHBP1 antibody
    see all


  • Anti-PUF60/FIR antibody (ab22819) at 0.1 µg/ml + HEK293 cell lysate (35µg in RIPA buffer)

    Predicted band size: 58 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human prostate staining PUF60/FIR with ab22819 at 5 μg/ml

  • ICC/IF image of ab22819 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22819, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skin staining PUF60/FIR with ab22819 at 5 μg/ml

  • Anti-PUF60/FIR antibody (ab22819) at 1 µg/ml + A431 lysate (35µg protein in RIPA buffer).

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.


This product has been referenced in:

  • Sun D  et al. PUF60 accelerates the progression of breast cancer through downregulation of PTEN expression. Cancer Manag Res 11:821-830 (2019). Read more (PubMed: 30697074) »
  • Corsini L  et al. Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60. J Biol Chem 284:630-9 (2009). WB . Read more (PubMed: 18974054) »
See all 2 Publications for this product

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