Key features and details
- Rabbit polyclonal to PUMA alpha - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PUMA alpha antibody - C-terminal
DescriptionRabbit polyclonal to PUMA alpha - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- NIH3T3, A549 and Hela cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 21 kDa.|
RelevancePUMA is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. PUMA is indeed an essential mediator of p53-dependent and -independent apoptosis in vivo, mediates the apoptotic response to p53 in colorectal cancer cells and is degraded during Chlamydia trachomatis infection
Cellular localizationCytoplasmic and Mitochondrial
- bbc3 antibody
- BCL2 binding component 3 antibody
- JFY1 antibody
All lanes : Anti-PUMA alpha antibody - C-terminal (ab182788) at 1/540 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Hela cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat anti Rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Exposure time: 1 minute
ab182788 has not yet been referenced specifically in any publications.