Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab109548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).|
FunctionEssential mediator of p53-dependent and p53-independent apoptosis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the Bcl-2 family.
Cellular localizationMitochondrion. Localized to the mitochondria in order to induce cytochrome c release.
- Information by UniProt
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
Anti-PUMA antibody (ab109548) at 0.1 µg/ml + Peripheral Blood Lymphocytes lysate at 35 µg
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
ab109548 has not yet been referenced specifically in any publications.