Product nameAnti-PUMA antibody
See all PUMA primary antibodies
DescriptionRabbit polyclonal to PUMA
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- This antibody gave a positive signal in MEF1 whole cell lysate and Human breast adenocarcinoma FFPE tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab26802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
FunctionEssential mediator of p53-dependent and p53-independent apoptosis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the Bcl-2 family.
Cellular localizationMitochondrion. Localized to the mitochondria in order to induce cytochrome c release.
- Information by UniProt
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
IHC image of ab26802 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26802, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-PUMA antibody (ab26802) at 2 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 40 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab26802 has not yet been referenced specifically in any publications.