Anti-PUMA antibody (ab9645)
Key features and details
- Rabbit polyclonal to PUMA
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PUMA antibody
See all PUMA primary antibodies -
Description
Rabbit polyclonal to PUMA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide:
ARARQEGSSPEPVEG
, corresponding to amino acids 2-16 of Human PUMA. (Peptide available as ab9646). -
Positive control
- WB: K562 lysate IHC-P: Human breast carcinoma sections ICC/IF: K562 cells
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General notes
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
|
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Essential mediator of p53-dependent and p53-independent apoptosis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the Bcl-2 family. -
Cellular localization
Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release. - Information by UniProt
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Database links
- Entrez Gene: 27113 Human
- Entrez Gene: 170770 Mouse
- Entrez Gene: 317673 Rat
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- SwissProt: Q99ML1 Mouse
- SwissProt: Q80ZG6 Rat
- Unigene: 467020 Human
see all -
Alternative names
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
Images
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Western blot - Anti-PUMA antibody (ab9645)Anti-PUMA antibody (ab9645) at 2 µg/ml + K562 cell lysates at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation: 1 hour at room temperature in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling PUMA with ab9645 at 10 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling PUMA with ab9645 at 2.5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9645)Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9645)Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab9645 has been referenced in 11 publications.
- Dong L & Vaux DL Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1. Cell Death Dis 11:442 (2020). PubMed: 32513923
- Jin S et al. 5-Azacitidine Induces NOXA to Prime AML Cells for Venetoclax-Mediated Apoptosis. Clin Cancer Res 26:3371-3383 (2020). PubMed: 32054729
- Mensink M et al. Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells. Cell Death Differ 25:795-806 (2018). WB ; Mouse . PubMed: 29339775
- Palomo GM et al. Silencing of frataxin gene expression triggers p53-dependent apoptosis in human neuron-like cells. Hum Mol Genet 20:2807-22 (2011). PubMed: 21531789
- Höglund A et al. Myc sensitizes p53-deficient cancer cells to the DNA-damaging effects of the DNA methyltransferase inhibitor decitabine. Blood 113:4281-8 (2009). WB, IF ; Human, Mouse . PubMed: 19179467
