Overview

  • Product name
    Anti-PUMA antibody
    See all PUMA primary antibodies
  • Description
    Rabbit polyclonal to PUMA
  • Host species
    Rabbit
  • Specificity
    Specific to PUMA alpha
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    ARARQEGSSPEPVEG

    , corresponding to amino acids 2-16 of Human PUMA. (Peptide available as ab9646).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • K562 lysate, human breast carcinoma sections
  • General notes


    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Properties

Applications

Our Abpromise guarantee covers the use of ab9645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa.Can be blocked with Human PUMA peptide (ab9646).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

Images

  • Western blot - Anti-PUMA antibody (ab9645)
    Western blot - Anti-PUMA antibody (ab9645)
    Anti-PUMA antibody (ab9645) at 2 µg/ml + human K562 cell lysate

    Performed under reducing conditions.


    Western blot analysis of PUMA expression in human K562 cell lysate with anti-PUMA at 2 µg /ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)
    Immunohistochemistry of PUMA in human breast carcinoma with ab9645 at 10 ug/ml.

References

This product has been referenced in:
  • Mensink M  et al. Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells. Cell Death Differ 25:795-806 (2018). WB ; Mouse . Read more (PubMed: 29339775) »
  • Palomo GM  et al. Silencing of frataxin gene expression triggers p53-dependent apoptosis in human neuron-like cells. Hum Mol Genet 20:2807-22 (2011). Read more (PubMed: 21531789) »

See all 9 Publications for this product

Publishing research using ab9645? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

1 Review or Q&A

10
Votes: Highest First

Thank you for getting back to me. We understand that you cannot give us a sample of an antibody to test. We had been using ab9643, but in light of our and other labs problems with the antibody, we are looking for a new PUMA antibody, specifically ab1...

Read More

I'm sorry, but I can not give out that type of information. We strongly encourage our customers to give us feedback on our antibodies and when we do receive such feedback, we post those reviews on the appropriate datasheets. Unfortunately in the case o...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up