Anti-PUMA antibody (ab9645)
- Datasheet
- References (9)
- Protocols
Overview
-
Product nameAnti-PUMA antibody
See all PUMA primary antibodies -
DescriptionRabbit polyclonal to PUMA
-
Host speciesRabbit
-
SpecificitySpecific to PUMA alpha
-
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide:
ARARQEGSSPEPVEG
, corresponding to amino acids 2-16 of Human PUMA. (Peptide available as ab9646). -
Positive control
- K562 lysate, human breast carcinoma sections
-
General notes
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium azide
-
Concentration information loading... -
PurityImmunogen affinity purified
-
Primary antibody notesApoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa.Can be blocked with Human PUMA peptide (ab9646). | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. |
Target
-
FunctionEssential mediator of p53-dependent and p53-independent apoptosis.
-
Tissue specificityUbiquitously expressed.
-
Sequence similaritiesBelongs to the Bcl-2 family.
-
Cellular localizationMitochondrion. Localized to the mitochondria in order to induce cytochrome c release.
- Information by UniProt
-
Database links
- Entrez Gene: 27113 Human
- Entrez Gene: 170770 Mouse
- Entrez Gene: 317673 Rat
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- SwissProt: Q99ML1 Mouse
- SwissProt: Q80ZG6 Rat
- Unigene: 467020 Human
see all -
Alternative names
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
Images
-
Western blot - Anti-PUMA antibody (ab9645)Anti-PUMA antibody (ab9645) at 2 µg/ml + human K562 cell lysate
Performed under reducing conditions.Western blot analysis of PUMA expression in human K562 cell lysate with anti-PUMA at 2
µ g /ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)Immunohistochemistry of PUMA in human breast carcinoma with ab9645 at 10 ug/ml.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Mensink M et al. Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells. Cell Death Differ 25:795-806 (2018). WB ; Mouse . Read more (PubMed: 29339775) »
- Palomo GM et al. Silencing of frataxin gene expression triggers p53-dependent apoptosis in human neuron-like cells. Hum Mol Genet 20:2807-22 (2011). Read more (PubMed: 21531789) »
