Anti-PUMA antibody (ab9645)
Key features and details
- Rabbit polyclonal to PUMA
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PUMA antibody
See all PUMA primary antibodies -
Description
Rabbit polyclonal to PUMA -
Host species
Rabbit -
Specificity
Specific to PUMA alpha -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide:
ARARQEGSSPEPVEG
, corresponding to amino acids 2-16 of Human PUMA. (Peptide available as ab9646). -
Positive control
- WB: K562 lysate IHC-P: Human breast carcinoma sections ICC/IF: K562 cells
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General notes
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa.
|
|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
|
| IHC-P |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Essential mediator of p53-dependent and p53-independent apoptosis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the Bcl-2 family. -
Cellular localization
Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release. - Information by UniProt
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Database links
- Entrez Gene: 27113 Human
- Entrez Gene: 170770 Mouse
- Entrez Gene: 317673 Rat
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- SwissProt: Q99ML1 Mouse
- SwissProt: Q80ZG6 Rat
- Unigene: 467020 Human
see all -
Alternative names
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
Images
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Western blot - Anti-PUMA antibody (ab9645)Anti-PUMA antibody (ab9645) at 2 µg/ml + K562 cell lysates at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation: 1 hour at room temperature in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling PUMA with ab9645 at 10 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling PUMA with ab9645 at 2.5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9645)Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9645)Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab9645 has been referenced in 9 publications.
- Mensink M et al. Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells. Cell Death Differ 25:795-806 (2018). WB ; Mouse . PubMed: 29339775
- Palomo GM et al. Silencing of frataxin gene expression triggers p53-dependent apoptosis in human neuron-like cells. Hum Mol Genet 20:2807-22 (2011). PubMed: 21531789
- Höglund A et al. Myc sensitizes p53-deficient cancer cells to the DNA-damaging effects of the DNA methyltransferase inhibitor decitabine. Blood 113:4281-8 (2009). WB, IF ; Human, Mouse . PubMed: 19179467
- Lim LY et al. Mutant p53 mediates survival of breast cancer cells. Br J Cancer 101:1606-12 (2009). WB ; Human . PubMed: 19773755
- Harford TJ et al. Increased expression of the pro-apoptotic Bcl2 family member PUMA and apoptosis by the muscle regulatory transcription factor MyoD in response to a variety of stimuli. Apoptosis : (2009). WB ; Mouse . PubMed: 19943111
- Leong CO et al. The p63/p73 network mediates chemosensitivity to cisplatin in a biologically defined subset of primary breast cancers. J Clin Invest 117:1370-80 (2007). PubMed: 17446929
- Bouchard C et al. FoxO transcription factors suppress Myc-driven lymphomagenesis via direct activation of Arf. Genes Dev 21:2775-87 (2007). PubMed: 17974917
- Biswas SC et al. Puma and p53 play required roles in death evoked in a cellular model of Parkinson disease. Neurochem Res 30:839-45 (2005). PubMed: 16187218
- Stankovic T et al. Microarray analysis reveals that TP53- and ATM-mutant B-CLLs share a defect in activating proapoptotic responses after DNA damage but are distinguished by major differences in activating prosurvival responses. Blood 103:291-300 (2004). PubMed: 12958068