Overview

  • Product name

    Anti-PUMA antibody - C-terminal
    See all PUMA primary antibodies
  • Description

    Rabbit polyclonal to PUMA - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PUMA (C terminal). 14 amino acids long. This sequence is identical between alpha and beta forms of the PUMA proteins.
    Database link: Q9BXH1

  • Positive control

    • Human breast and small intestine tissues; K562 cells; K562 and 3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab189123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 2 µg/ml.

Target

  • Function

    Essential mediator of p53-dependent and p53-independent apoptosis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Cellular localization

    Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release.
  • Information by UniProt
  • Database links

  • Alternative names

    • BBC 3 antibody
    • Bbc3 antibody
    • BBC3_HUMAN antibody
    • BCL 2 binding component 3 antibody
    • Bcl-2-binding component 3 antibody
    • BCL2 binding component 3 antibody
    • JFY 1 antibody
    • JFY-1 antibody
    • JFY1 antibody
    • p53 up regulated modulator of apoptosis antibody
    • p53 up-regulated modulator of apoptosis antibody
    • p53 Upregulated Modulator of Apoptosis antibody
    • PUMA alpha antibody
    • PUMA/JFY1 antibody
    see all

Images

  • All lanes : Anti-PUMA antibody - C-terminal (ab189123) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : 3T3 cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling PUMA with ab189123 at 10 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling PUMA with ab189123 at 10 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of K562 cells labeling PUMA with ab189123 at 2 µg/ml.

  • Immunocytochemical analysis of K562 cells labeling PUMA with ab189123 at 1 µg/ml.

References

ab189123 has not yet been referenced specifically in any publications.

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