Overview

  • Product name

    Anti-PUMA antibody - C-terminal
    See all PUMA primary antibodies
  • Description

    Rabbit polyclonal to PUMA - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PUMA (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q9BXH1

  • Positive control

    • WB: HCT 116 treated with cisplatin whole cell lysate; K562 and THP-1 whole cell extracts. IP: HCT 116 treated with cisplatin whole cell extract. IHC-P: Human hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Essential mediator of p53-dependent and p53-independent apoptosis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Cellular localization

    Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release.
  • Information by UniProt
  • Database links

  • Alternative names

    • BBC 3 antibody
    • Bbc3 antibody
    • BBC3_HUMAN antibody
    • BCL 2 binding component 3 antibody
    • Bcl-2-binding component 3 antibody
    • BCL2 binding component 3 antibody
    • JFY 1 antibody
    • JFY-1 antibody
    • JFY1 antibody
    • p53 up regulated modulator of apoptosis antibody
    • p53 up-regulated modulator of apoptosis antibody
    • p53 Upregulated Modulator of Apoptosis antibody
    • PUMA alpha antibody
    • PUMA/JFY1 antibody
    see all

Images

  • All lanes : Anti-PUMA antibody - C-terminal (ab227130) at 1/1000 dilution

    Lane 1 : HCT 116 (human colorectal carcinoma cell line) with mock treatment for 0 hours, whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) with 30 uM cisplatin treatment for 24 hours, whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    15% SDS-PAGE gel.

  • All lanes : Anti-PUMA antibody - C-terminal (ab227130) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    12 % SDS-PAGE gel.

  • PUMA was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 24 hours, whole cell extract with 4 µg of ab227130. Western blot was performed from the immunoprecipitate using ab227130. Anti-rabbit IgG was used as a secondary reagent.

    Lane 1: Preimmune Rabbit IgG instead of ab227130 in HCT 116 treated with 30 µM cisplatin for 24 hours, whole cell extract.

    Lane 2: ab227130 IP in HCT 116 treated with 30 µM cisplatin for 24 hours, whole cell extract.

    15 % SDS-PAGE gel.

  • Paraffin-embedded human hepatoma tissue tissue stained for PUMA using ab227130 at 1/500 dilution in immunohistochemical analysis.

References

ab227130 has not yet been referenced specifically in any publications.

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