Anti-Puma gamma/GPR109A antibody [MM0329-6S24] (ab89298)
Key features and details
- Rat monoclonal [MM0329-6S24] to Puma gamma/GPR109A
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2
Overview
-
Product name
Anti-Puma gamma/GPR109A antibody [MM0329-6S24]
See all Puma gamma/GPR109A primary antibodies -
Description
Rat monoclonal [MM0329-6S24] to Puma gamma/GPR109A -
Host species
Rat -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Puma gamma/GPR109A.
-
Positive control
- WB: Human placenta tissue lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography. -
Clonality
Monoclonal -
Clone number
MM0329-6S24 -
Isotype
IgG2 -
Research areas
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 44 kDa).
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 44 kDa). |
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Function
Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. -
Tissue specificity
Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Developmental stage
Expression in neutrophils occurs in the late terminal differentiation phase. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 338442 Human
- Omim: 609163 Human
- SwissProt: Q8TDS4 Human
- Unigene: 524812 Human
-
Alternative names
- G protein coupled receptor 109A antibody
- G protein coupled receptor HM74a antibody
- G protein coupled receptor Hm74b antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (0)
ab89298 has not yet been referenced specifically in any publications.