Overview

  • Product name

    Anti-Puma gamma/GPR109A antibody
    See all Puma gamma/GPR109A primary antibodies
  • Description

    Rabbit polyclonal to Puma gamma/GPR109A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Puma gamma/GPR109A aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101077)

  • Positive control

    • This antibody gave a positive signal in Human liver tissue lysate.
  • General notes

     This product was previously labelled as Puma gamma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab81825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
  • Tissue specificity

    Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Developmental stage

    Expression in neutrophils occurs in the late terminal differentiation phase.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor 109A antibody
    • G protein coupled receptor HM74a antibody
    • G protein coupled receptor Hm74b antibody
    • G-protein coupled receptor 109A antibody
    • G-protein coupled receptor HM74A antibody
    • G109A_HUMAN antibody
    • GPR 109A antibody
    • Gpr109a antibody
    • GPR109A protein antibody
    • HCA2 antibody
    • HCAR2 antibody
    • HM 74 antibody
    • HM 74a antibody
    • HM 74b antibody
    • Hm74 antibody
    • HM74a antibody
    • HM74b antibody
    • hydroxycarboxylic acid receptor 2 antibody
    • Interferon gamma inducible gene Puma g antibody
    • Niacin receptor 1 antibody
    • NIACR1 antibody
    • Nicotinic acid receptor 1 antibody
    • Nicotinic acid receptor antibody
    • Protein upregulated in macrophages by IFNG, mouse, homolog of antibody
    • Puma g antibody
    • Pumag antibody
    • rHM74b antibody
    see all

Images

  • Anti-Puma gamma/GPR109A antibody (ab81825) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 12 minutes
  • ICC/IF image of ab81825 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81825, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 rabbit anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Zhou D  et al. Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression. Exp Mol Med 50:157 (2018). Read more (PubMed: 30510243) »
See 1 Publication for this product

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