Anti-Pumilio 1 antibody (ab3717)

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Overview

  • Product name

    Anti-Pumilio 1 antibody
    See all Pumilio 1 primary antibodies

  • Description

    Goat polyclonal to Pumilio 1

  • Host species

    Goat

  • Tested applications

    Suitable for: IHC-Fr, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant

  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

  • Positive control

    • HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21865157
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
IP Use at 1-5 µg/mg of lysate.

Target

Images

  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)

    Sample: Hela cell total RIPA extract (10µg)

    Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

    CIP: Calf Intestinal Phosphatase

    Detection: ECL exposure for 1 minute.

    Sample: Hela cell total RIPA extract (10µg)

    Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

    CIP: Calf Intestinal Phosphatase

    Detection: ECL exposure for 1 minute.

References

This product has been referenced in:

  • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). Read more (PubMed: 30269240) »
  • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). Read more (PubMed: 30197756) »
See all 3 Publications for this product

Publishing research using ab3717? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Customer's question: Do you know whether ab3717 cross reacts with Pum2. If you do not know, could you send me the peptide sequence to see if it is likely to cross react. Thank you

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Answer

ab3717 does not cross react with Pum2.

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