Anti-Pumilio 1 antibody (ab3717)
Key features and details
- Goat polyclonal to Pumilio 1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Pumilio 1 antibody
See all Pumilio 1 primary antibodies -
Description
Goat polyclonal to Pumilio 1 -
Host species
Goat -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.
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Positive control
- WB: NIH/3T3, HEK293T, A549, K562 whole cell lysate; IP: HEK293T
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
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| Notes |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 535450 Cow
- Entrez Gene: 9698 Human
- Entrez Gene: 80912 Mouse
- Entrez Gene: 362609 Rat
- Omim: 607204 Human
- SwissProt: Q14671 Human
- SwissProt: Q80U78 Mouse
- SwissProt: Q5R5X3 Orangutan
see all -
Form
Cytoplasm -
Alternative names
- HsPUM antibody
- KIAA0099 antibody
- PUM 1 antibody
see all
Images
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Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 µg/mL.
Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.
Lane 2: ab3717 IP in HEK293T whole cell lysate.
Lane 3: ab3717 IP in HEK293T whole cell lysate
Lane 4: Control IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds
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Western blot - Anti-Pumilio 1 antibody (ab3717)All lanes : Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated rabbit anti-goat IgG
Predicted band size: 126 kDa
Exposure time: 10 seconds -
Western blot - Anti-Pumilio 1 antibody (ab3717)Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Secondary
HRP-conjugated rabbit anti-goat IgG
Predicted band size: 126 kDa
Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab3717 has been referenced in 3 publications.
- Sajek M et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
- Janecki DM et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
- Marrero E et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157