Overview

  • Product name

  • Description

    Goat polyclonal to Pumilio 1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

  • Positive control

    • HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21865157
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
IP Use at 1-5 µg/mg of lysate.

Target

Images

  • Sample: Hela cell total RIPA extract (10µg)

    Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

    CIP: Calf Intestinal Phosphatase

    Detection: ECL exposure for 1 minute.

    Sample: Hela cell total RIPA extract (10µg)

    Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

    CIP: Calf Intestinal Phosphatase

    Detection: ECL exposure for 1 minute.

References

This product has been referenced in:

  • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). Read more (PubMed: 30269240) »
  • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). Read more (PubMed: 30197756) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

ab3717 does not cross react with Pum2.

Read More

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