Anti-Pumilio 1 antibody (ab3717)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Pumilio 1 antibody
See all Pumilio 1 primary antibodies -
DescriptionGoat polyclonal to Pumilio 1
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Host speciesGoat
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Tested applicationsSuitable for: IHC-Fr, WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.
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Positive control
- HeLa cell extract.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21865157 | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa). | |
| IP | Use at 1-5 µg/mg of lysate. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 535450 Cow
- Entrez Gene: 9698 Human
- Entrez Gene: 80912 Mouse
- Entrez Gene: 362609 Rat
- Omim: 607204 Human
- SwissProt: Q14671 Human
- SwissProt: Q80U78 Mouse
- SwissProt: Q5R5X3 Orangutan
see all -
FormCytoplasm
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Alternative names
- HsPUM antibody
- KIAA0099 antibody
- PUM 1 antibody
see all
Images
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Western blot - Anti-Pumilio 1 antibody (ab3717)Sample: Hela cell total RIPA extract (10
µ g)Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)
CIP: Calf Intestinal Phosphatase
Detection: ECL exposure for 1 minute.
Sample: Hela cell total RIPA extract (10
µg) Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)
CIP: Calf Intestinal Phosphatase
Detection: ECL exposure for 1 minute.
References
This product has been referenced in:
- Marrero E et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21865157) »
