Anti-Pumilio 1 antibody (ab3717)

Submit a review Q&A (1)References (3)
Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
  • Western blot - Anti-Pumilio 1 antibody (ab3717)
  • Western blot - Anti-Pumilio 1 antibody (ab3717)

Key features and details

  • Goat polyclonal to Pumilio 1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Pumilio 1 antibody
    See all Pumilio 1 primary antibodies

  • Description

    Goat polyclonal to Pumilio 1

  • Host species

    Goat

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant

  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

  • Positive control

    • WB: NIH/3T3, HEK293T, A549, K562 whole cell lysate; IP: HEK293T

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

Target

Images

  • Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
    Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)

    Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 µg/mL.

    Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.

    Lane 2: ab3717 IP in HEK293T whole cell lysate.

    Lane 3: ab3717 IP in HEK293T whole cell lysate

    Lane 4: Control IgG in HEK293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 seconds

  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)
    All lanes : Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : A549 whole cell lysate
    Lane 3 : K562 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated rabbit anti-goat IgG

    Predicted band size: 126 kDa


    Exposure time: 10 seconds
  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)
    Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml + NIH/3T3 whole cell lysate at 50 µg

    Secondary
    HRP-conjugated rabbit anti-goat IgG

    Predicted band size: 126 kDa


    Exposure time: 10 seconds

References (3)

Publishing research using ab3717? Please let us know so that we can cite the reference in this datasheet.

ab3717 has been referenced in 3 publications.

  • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
  • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
  • Marrero E  et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157

Customer reviews and Q&As

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Question

Customer's question: Do you know whether ab3717 cross reacts with Pum2. If you do not know, could you send me the peptide sequence to see if it is likely to cross react. Thank you

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Answer

ab3717 does not cross react with Pum2.

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