Product nameAnti-Pumilio 1 antibody
See all Pumilio 1 primary antibodies
DescriptionRabbit polyclonal to Pumilio 1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Chicken, Human, Macaque monkey, Orangutan
Synthetic peptide corresponding to Mouse Pumilio 1 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse lung tissue lysate in western blot and MCF7 whole cells in Immunofluorescence.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 127 kDa).|
- Information by UniProt
- HsPUM antibody
- KIAA0099 antibody
- PUM 1 antibody
ICC/IF image of ab97993 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97993 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa, Hek293,and HepG2 cells at 5ug/ml.
Anti-Pumilio 1 antibody (ab97993) at 1 µg/ml +
Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 147 kDa why is the actual band size different from the predicted?
Additional bands at: 250 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The predicted molecular weight of Pumilio 1 is 127 kDa (SwissProt), however we expect to observe a banding pattern around 150 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab97993 has not yet been referenced specifically in any publications.