RecombinantRabMAb

Recombinant Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3795] to Pumilio 1 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free
    See all Pumilio 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3795] to Pumilio 1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is predicted to not cross react with Pumilio 2.

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Pumilio 1 aa 150-250. The exact sequence is proprietary.
    Database link: Q14671

  • General notes

    ab247584 is the carrier-free version of ab92545 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247584 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

     

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This antibody may not be suitable for IHC with mouse or rat samples.

 
WB Use at an assay dependent concentration. Predicted molecular weight: 126 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Pumilio 1 with Purified ab92545 at 1:100 dilution (1.18 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

      Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Pumilio 1 with purified ab92545 at 1:20 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 1 with purified ab92545 at 1:500 dilution (0.2μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 126 kDa



      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using an unpurified ab92545 at a 1/100 dilution.
    • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution (purified)

      Lane 1 : Mouse cerebellum tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 126 kDa



      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/5000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 126 kDa



      This data was developed using ab92545, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      This data was developed using ab92545, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Pumilio 1 in HeLa cells using an unpurified ab92545 at a 1/100 dilution.
    • Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      This data was developed using ab92545, the same antibody clone in a different buffer formulation.Overlay histogram showing HEK293 cells stained with an unpurified ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
      Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247584? Please let us know so that we can cite the reference in this datasheet.

    ab247584 has not yet been referenced specifically in any publications.

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