• Product name

  • Description

    Rabbit polyclonal to Pumilio 2
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human Pumilio homolog 2 encoded within exon 4 (LocusLink ID 23369).

  • Positive control

    • Whole cell lysate from a human monocytic KG-1 cell line, mouse multipotential hematopoietic EML cell line or mouse pre-B 70 Z/3 cell line. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.



Our Abpromise guarantee covers the use of ab10361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 127 kDa).
IP Use at 5-10 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 21865157
ICC/IF Use a concentration of 5 µg/ml.


  • Function

    Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificity

    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similarities

    Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • Domain

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localization

    Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all


  • ab10361 detection of human and mouse Pumilio 2 by Western blot. Samples: Whole cell lysate (50 mg) from a human monocytic (KG-1; lane 1), mouse multipotential hematopoietic (EML; lane 2) or mouse pre-B (70 Z/3; lane 3) cell lines. Antibody: ab10361 used at 0.1 mg/ml. Detection: Chemiluminescence with 3 to 5 minute exposure.

    ab10361 detection of human and mouse Pumilio 2 by Western blot. Samples: Whole cell lysate (50 mg) from a human monocytic (KG-1; lane 1), mouse multipotential hematopoietic (EML; lane 2) or mouse pre-B (70 Z/3; lane 3) cell lines. Antibody: ab10361 used at 0.1 mg/ml. Detection: Chemiluminescence with 3 to 5 minute exposure.
  • ICC/IF image of ab10361 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10361 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Wang Y  et al. PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression. Cell Struct Funct 44:29-39 (2019). Read more (PubMed: 30787206) »
  • Khong A  et al. The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules. Mol Cell 68:808-820.e5 (2017). Read more (PubMed: 29129640) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


ab57644, anti-RPS23, code de remise : ******** ab10361, anti-Pumilio 2, code de remise : ******** Date d’expiration pour ces deux codes : JJ MM AAAA. Je suis ravi que vous souhaitiez participer à notre offre et tester ab10361 and ab57644 chez l'oursin. Les codes de réduction ci-dessus sont utilisables sous 4 mois et pour l’achat d'autres anticorps primaires. Vous devez dans un premier temps soumettre une Abreview avec vos résultats chez l'oursin pour chacun de ces 2 anticorps en notant le code promotionnel dans la section « Additional notes ». Le code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de l'anticorps testé. Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches. Termes et Conditions : www.abcam.com/collaborationdiscount.

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Thank you for contacting Abcam. As we do not know the exact immunogen sequence for ab10361 and the similarity between the human and sea urchin is not high, I would not suggest that particular antibody. I would however recommend trying ab57049, as we know the exact immunogen sequence for this antibody and when I do an alignment with the sequence you provided, there is a 91.5% homology between the two. Therefore, although I cannot guarantee that it will work, I am confident. Please let me know if there is anything else I can help you with.

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Human Cell lysate - whole cell (HEK 293T)
HEK 293T
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C antibody (sc-15385) bound protein A dynabeads 0.1 mg/ml (antibody/beads).
Negative control
Protein A dynabeads without antibody bound.

Abcam user community

Verified customer

Submitted Aug 18 2011


Thank you for your enquiry and your interest in our products. We do apologize but the immunizing peptides are not available commercially.

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