Key features and details
- Rabbit polyclonal to Pumilio 2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pumilio 2 antibody
See all Pumilio 2 primary antibodies
DescriptionRabbit polyclonal to Pumilio 2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Pumilio 2 conjugated to Keyhole Limpet Haemocyanin (KLH). surrounding S182. NP_056132.1.
Database link: Q8TB72-3
- Human heart and Human prostate carcinoma tissues; HepG2 cells; 293 cell lysates, transiently transfected with the Pumilio 2 gene.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190104 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 114 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Tissue specificityExpressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
Sequence similaritiesContains 1 PUM-HD domain.
Contains 8 pumilio repeats.
DomainThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
Cellular localizationCytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
- Information by UniProt
- FLJ36528 antibody
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All lanes : Anti-Pumilio 2 antibody (ab190104) at 1/1000 dilution
Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate, transiently transfected with the Pumilio 2 gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 114 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Pumilio 2 with ab190104 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart labeling Pumilio 2 with ab190104 at 10 µg/ml.
Flow cytometric analysis of HepG2 cells labeling Pumilio 2 with ab190104 at 1/10 dilution (lower image) compared with a negative control (upper image). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab190104 has not yet been referenced specifically in any publications.