Overview

  • Product name

  • Description

    Rabbit polyclonal to Pumilio 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Pumilio 2 conjugated to Keyhole Limpet Haemocyanin (KLH). surrounding S182. NP_056132.1.
    Database link: Q8TB72-3

  • Positive control

    • Human heart and Human prostate carcinoma tissues; HepG2 cells; 293 cell lysates, transiently transfected with the Pumilio 2 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab190104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 114 kDa.
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificity

    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similarities

    Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • Domain

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localization

    Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all

Images

  • All lanes : Anti-Pumilio 2 antibody (ab190104) at 1/1000 dilution

    Lane 1 : 293 cell lysate, non transfected
    Lane 2 : 293 cell lysate, transiently transfected with the Pumilio 2 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 114 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Pumilio 2 with ab190104 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart labeling Pumilio 2 with ab190104 at 10 µg/ml.

  • Flow cytometric analysis of HepG2 cells labeling Pumilio 2 with ab190104 at 1/10 dilution (lower image) compared with a negative control (upper image).  FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab190104 has not yet been referenced specifically in any publications.

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