• Product name

  • Description

    Goat polyclonal to Pumilio 2
  • Host species

  • Specificity

    ab32894 recognises PUM2.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee
  • Immunogen

    Synthetic peptide:


    , corresponding to Internal sequence amino acids 141-155 of Human Pumilio 2

  • Positive control

    • Human Epstein-Barr virus immortilised lymphoblastoid lysate.



Our Abpromise guarantee covers the use of ab32894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
WB Use a concentration of 0.3 - 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 114 kDa).
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificity

    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similarities

    Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • Domain

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localization

    Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human testis staining Pumilio 2 with ab32894 at 10 μg/ml

  • All lanes : Anti-Pumilio 2 antibody (ab32894) at 1 µg/ml

    Lane 1 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - without blocking with the immunizing peptide
    Lane 2 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - blocking with the immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 114 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa, 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    NB: Some minor bands are resistant to blocking by the immunizing peptide.
  • Lane 1 : Anti-Pumilio 2 antibody (ab32894) at 0.5 µg/ml
    Lane 2 : Anti-Pumilio 2 antibody (ab32894) at 1 mg/ml
    Lane 3 : anti- DYKDDDDK Tag at 1/3000 dilution

    Lanes 1 & 3 : HEK293 lysate overexpressing Human PUM2 with DYKDDDDK tag at 10 µg
    Lane 2 : Mock Tansfected HEK293

    Predicted band size: 114 kDa

    Primary Incubation time 1 hour


ab32894 has not yet been referenced specifically in any publications.

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