Key features and details
- Rabbit polyclonal to Pumilio 2 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Pumilio 2 antibody - C-terminal
See all Pumilio 2 primary antibodies
DescriptionRabbit polyclonal to Pumilio 2 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Medaka fish
Recombinant fragment within Human Pumilio 2 (C terminal). The exact sequence is proprietary.
Database link: Q8TB72
- WB: Mouse ovary tissue extract; Human embryonic stem cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 114 kDa.|
FunctionSequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Tissue specificityExpressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
Sequence similaritiesContains 1 PUM-HD domain.
Contains 8 pumilio repeats.
DomainThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
Cellular localizationCytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
- Information by UniProt
- FLJ36528 antibody
- KIAA0235 antibody
- MGC138251 antibody
Anti-Pumilio 2 antibody - C-terminal (ab228787) at 1/500 dilution + Mouse ovary tissue extract at 50 µg
Predicted band size: 114 kDa
7.5% SDS-PAGE gel.
Anti-Pumilio 2 antibody - C-terminal (ab228787) at 1/2000 dilution + Human embryonic stem cell lysate at 20 µg
Predicted band size: 114 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228787 has not yet been referenced specifically in any publications.