Overview

  • Product name

    Anti-Pumilio 2 antibody [EPR3813]
    See all Pumilio 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3813] to Pumilio 2
  • Host species

    Rabbit
  • Specificity

    This antibody is predicted to not cross react with Pumilio 1.
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pumilio 2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and 293T cell lysates ICC/IF: MCF-7 cells. FC: HeLa cells IP: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.
WB 1/5000 - 1/10000. Predicted molecular weight: 114 kDa.
IP 1/10 - 1/20.
ICC/IF 1/50.

For unpurified use at 1/100 - 1/250.

Target

  • Function

    Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificity

    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similarities

    Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • Domain

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localization

    Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all

Images

  • All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/50 dilution (4.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/30 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • ab92390 (purified) at 1/20 dilution (1ug) immunoprecipitating Pumilio 2 in HeLa whole cell lysates.
    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab92390 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92390 in HeLa whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

References

This product has been referenced in:

See all 6 Publications for this product

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