RecombinantRabMAb

Recombinant Anti-Pumilio 2 antibody [EPR3813] (ab92390)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3813] to Pumilio 2
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pumilio 2 antibody [EPR3813]
    See all Pumilio 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3813] to Pumilio 2

  • Host species

    Rabbit

  • Specificity

    This antibody is predicted to not cross react with Pumilio 1.

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Pumilio 2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and 293T cell lysates ICC/IF: MCF-7 cells. Flow Cyt: HeLa cells IP: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab92390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.
WB 1/5000 - 1/10000. Predicted molecular weight: 114 kDa.
IP 1/10 - 1/20.
ICC/IF 1/50.

For unpurified use at 1/100 - 1/250.

Target

  • Function

    Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

  • Tissue specificity

    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.

  • Sequence similarities

    Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.

  • Domain

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.

  • Cellular localization

    Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ36528 antibody
      • KIAA0235 antibody
      • MGC138251 antibody
      • MGC138253 antibody
      • PUM 2 antibody
      • PUM2 antibody
      • PUM2_HUMAN antibody
      • PUMH 2 antibody
      • PUMH2 antibody
      • Pumilio (Drosphila) homolog 2 antibody
      • Pumilio homolog 2 (Drosophila) antibody
      • Pumilio homolog 2 antibody
      • Pumilio-2 antibody
      • Pumilio2 antibody
      • PUML2 antibody
      see all

    Images

    • Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/10000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 114 kDa
      Observed band size: 114 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      Immunocytochemistry/ Immunofluorescence - Anti-Pumilio 2 antibody [EPR3813] (ab92390)

      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/50 dilution (4.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      Flow Cytometry - Anti-Pumilio 2 antibody [EPR3813] (ab92390)

      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/30 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunoprecipitation - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      Immunoprecipitation - Anti-Pumilio 2 antibody [EPR3813] (ab92390)

      ab92390 (purified) at 1/20 dilution (1ug) immunoprecipitating Pumilio 2 in HeLa whole cell lysates.
      Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
      Lane 2 (+): ab92390 & HeLa whole cell lysates
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92390 in HeLa whole cell lysates
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 114 kDa
      Observed band size: 114 kDa

    • Anti-Pumilio 2 antibody [EPR3813] (ab92390)
      Anti-Pumilio 2 antibody [EPR3813] (ab92390)

    References (7)

    Publishing research using ab92390? Please let us know so that we can cite the reference in this datasheet.

    ab92390 has been referenced in 7 publications.

    • Cao Y  et al. Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. Exp Cell Res 393:112058 (2020). PubMed: 32437714
    • Elguindy MM  et al. PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD. Elife 8:N/A (2019). PubMed: 31343408
    • Kopp F  et al. PUMILIO hyperactivity drives premature aging of Norad-deficient mice. Elife 8:N/A (2019). PubMed: 30735131
    • Lin K  et al. Essential requirement of mammalian Pumilio family in embryonic development. Mol Biol Cell 29:2922-2932 (2018). PubMed: 30256721
    • Hubstenberger A  et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons. Mol Cell 68:144-157.e5 (2017). PubMed: 28965817
    • Zhang M  et al. Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins. Genes Dev N/A:N/A (2017). PubMed: 28794184
    • Mak W  et al. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline. Biol Reprod 94:134 (2016). PubMed: 27170441

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