Recombinant
Anti-Pumilio 2 antibody [EPR3813] (ab92390)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Pumilio 2 antibody [EPR3813]
See all Pumilio 2 primary antibodies -
DescriptionRabbit monoclonal [EPR3813] to Pumilio 2
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Host speciesRabbit
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SpecificityThis antibody is predicted to not cross react with Pumilio 1.
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Tested applicationsSuitable for: WB, IP, ICCmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Pumilio 2 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa and 293T cell lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3813
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab92390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| ICC |
Application notes
ICC: 1/100 - 1/250.
IP: 1/10.
WB: 1/5000 - 1/10000. Predicted molecular weight: 114 kDa.
Is unsuitable for Flow Cyt or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/10.
WB: 1/5000 - 1/10000. Predicted molecular weight: 114 kDa.
Is unsuitable for Flow Cyt or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionSequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
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Tissue specificityExpressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
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Sequence similaritiesContains 1 PUM-HD domain.
Contains 8 pumilio repeats. -
DomainThe pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
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Cellular localizationCytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
- Information by UniProt
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Database links
- Entrez Gene: 23369 Human
- Entrez Gene: 80913 Mouse
- Entrez Gene: 298874 Rat
- Omim: 607205 Human
- SwissProt: Q8TB72 Human
- SwissProt: Q80U58 Mouse
- Unigene: 467824 Human
- Unigene: 341243 Mouse
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Alternative names
- FLJ36528 antibody
- KIAA0235 antibody
- MGC138251 antibody
see all
Images
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![Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)](https://www.abcam.com/ps/products/92/ab92390/Images/Pumilio-2-Primary-antibodies-ab92390-2.gif)
Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
Protocols
References
This product has been referenced in:
- Zhang M et al. Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins. Genes Dev N/A:N/A (2017). Read more (PubMed: 28794184) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"