Overview

  • Product name

    Anti-Pumilio 2 antibody - N-terminal
    See all Pumilio 2 primary antibodies
  • Description

    Rabbit polyclonal to Pumilio 2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Pumilio 2 (N terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: Q8TB72

  • Positive control

    • WB: Human ESC whole cell lysate; HEK-293T, A431 and HeLa whole cell extracts. IHC-P: Mouse kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 114 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
  • Tissue specificity

    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
  • Sequence similarities

    Contains 1 PUM-HD domain.
    Contains 8 pumilio repeats.
  • Domain

    The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
  • Cellular localization

    Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36528 antibody
    • KIAA0235 antibody
    • MGC138251 antibody
    • MGC138253 antibody
    • PUM 2 antibody
    • PUM2 antibody
    • PUM2_HUMAN antibody
    • PUMH 2 antibody
    • PUMH2 antibody
    • Pumilio (Drosphila) homolog 2 antibody
    • Pumilio homolog 2 (Drosophila) antibody
    • Pumilio homolog 2 antibody
    • Pumilio-2 antibody
    • Pumilio2 antibody
    • PUML2 antibody
    see all

Images

  • Paraffin-embedded mouse kidney tissue stained for Pumilio 2 with ab228743 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Pumilio 2 antibody - N-terminal (ab228743) at 1/5000 dilution + Human ESC whole cell lysate at 20 µg

    Developed using the ECL technique.

    Predicted band size: 114 kDa



    5% SDS-PAGE

  • All lanes : Anti-Pumilio 2 antibody - N-terminal (ab228743) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 114 kDa



    7.5% SDS-PAGE

References

ab228743 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228743.
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