Key features and details
- Rabbit polyclonal to Punt
- Suitable for: ICC, IP, WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Product nameAnti-Punt antibody
DescriptionRabbit polyclonal to Punt
Tested applicationsSuitable for: ICC, IP, WBmore details
Species reactivityReacts with: Drosophila melanogaster
- D. melanogaster extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
The antiserum is supplied in antibody stabilization buffer.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified against immobilized antigen based affinity chromatography which yielded an epitope specific antibody.
Our Abpromise guarantee covers the use of ab14680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: >1/500. Detects a band of approximately 63 kDa
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog and wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promotes its cell division in the female germline. The Signaling by TGF beta related factors requires ligand induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor is encoded by the thick veins (tkv) gene and the type II receptor is encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF beta/Dpp (Decapentaplegic) receptor. Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required to block the development of various organelles including salivary glands. These are Mothers against dpp (Mad), Medea (Med) and Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development. Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp dependent patterning process. No Dpp dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling.
- Activin receptor type II antibody
- Atr antibody
- Pun antibody
All lanes : Anti-Punt antibody (ab14680) at 1/1000 dilution
Lane 1 : 0-4 hour old EGFP shRNAi embryos (Control)
Lane 2 : 0-4 hour old put shRNAi embryos
Lysates/proteins at 30 µg per lane.
All lanes : Amersham ECL anti-Rabbit IgG, HRP conjugated at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 180 kDa (possible non-specific binding), 38 kDa (possible non-specific binding), 40 kDa (possible non-specific binding), 55 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)
Exposure time: 4 minutes
ab14680 has not yet been referenced specifically in any publications.