Anti-PURA antibody (ab79936)
Key features and details
- Rabbit polyclonal to PURA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PURA antibody
See all PURA primary antibodies -
Description
Rabbit polyclonal to PURA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep -
Immunogen
Synthetic peptide corresponding to Human PURA aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab88160) -
Positive control
- This antibody gave a positive signal in Human Brain Tissue Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. -
Sequence similarities
Belongs to the PUR DNA-binding protein family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5813 Human
- Entrez Gene: 19290 Mouse
- Omim: 600473 Human
- SwissProt: Q00577 Human
- SwissProt: P42669 Mouse
- Unigene: 443121 Human
- Unigene: 231802 Mouse
- Unigene: 482241 Mouse
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Alternative names
- PUR ALPHA antibody
- PUR1 antibody
- purA antibody
see all
Images
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Anti-PURA antibody (ab79936) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?PURA contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, other commercially available antibodies react with a target at approximately 42 kDa.
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ICC/IF image of ab79936 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79936, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293. HepG2, MCF-7 cells at 5µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab79936 has been referenced in 8 publications.
- Sun Y et al. PURa Promotes the Transcriptional Activation of PCK2 in Oesophageal Squamous Cell Carcinoma Cells. Genes (Basel) 11:N/A (2020). PubMed: 33142842
- Swinnen B et al. A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism. Acta Neuropathol 135:427-443 (2018). PubMed: 29302778
- Moldovan JB & Moran JV The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet 11:e1005121 (2015). PubMed: 25951186
- Di Salvio M et al. Pur-alpha functionally interacts with FUS carrying ALS-associated mutations. Cell Death Dis 6:e1943 (2015). PubMed: 26492376
- Rossi S et al. Nuclear accumulation of mRNAs underlies G4C2-repeat-induced translational repression in a cellular model of C9orf72 ALS. J Cell Sci 128:1787-99 (2015). PubMed: 25788698
- Hay CW et al. An Sp1 Modulated Regulatory Region Unique to Higher Primates Regulates Human Androgen Receptor Promoter Activity in Prostate Cancer Cells. PLoS One 10:e0139990 (2015). WB . PubMed: 26448047
- Schreiber J et al. Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic ?-actin levels. J Cell Biol 211:569-86 (2015). PubMed: 26527743
- Xu Z et al. Expanded GGGGCC repeat RNA associated with amyotrophic lateral sclerosis and frontotemporal dementia causes neurodegeneration. Proc Natl Acad Sci U S A 110:7778-83 (2013). WB . PubMed: 23553836