Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) (ab204706)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
- Sensitivity: 0.005 µU
Overview
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Product name
Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
See all Nucleoside phosphorylase kits -
Detection method
Fluorescent -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Enzyme activity -
Sensitivity
0.005 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) (ab204706) is an assay where hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K767 Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric). K767-100 is the same size as the 100 test size of ab204706.
Purine Nucleoside Phosphorylase (PNP, E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T- cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 10X PNP Assay Buffer 1 x 10ml Hypoxanthine Standard 1 x 100µl Inosine Substrate 1 x 200µl OxiRed Probe 1 x 200µl PNP Enzyme Mix 1 vial PNP Positive Control 1 vial -
Research areas
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Involvement in disease
Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children. -
Sequence similarities
Belongs to the PNP/MTAP phosphorylase family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Alternative names
- FLJ94043
- FLJ97288
- FLJ97312
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204706 has not yet been referenced specifically in any publications.