Overview

  • Product name

    Anti-PUS1 antibody [EPR13235(B)]
    See all PUS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13235(B)] to PUS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PUS1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y606

  • Positive control

    • 293T, HeLa, Jurkat and fetal brain lysate; Human kidney and Human brain tissues; HeLa and Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
    • Tissue specificity

      Widely expressed. High levels of expression found in brain and skeletal muscle.
    • Involvement in disease

      Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1) [MIM:600462]; also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow.
    • Sequence similarities

      Belongs to the tRNA pseudouridine synthase TruA family.
    • Cellular localization

      Mitochondrion and Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • A730013B20Rik antibody
      • DOBI antibody
      • MGC112655 antibody
      • MGC11268 antibody
      • MLASA antibody
      • MLASA1 antibody
      • mPus1p antibody
      • Pseudouridine synthase 1 antibody
      • Pseudouridylate synthase 1 antibody
      • PUS1 antibody
      • tRNA pseudouridine synthase A, mitochondrial antibody
      • tRNA pseudouridine(38-40) synthase antibody
      • tRNA pseudouridylate synthase I antibody
      • tRNA uridine isomerase I antibody
      • tRNA-uridine isomerase I antibody
      • TRUA_HUMAN antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labeling PUS1 with ab175240 at 1/100 (red) and DAPI staining (blue).

    • All lanes : Anti-PUS1 antibody [EPR13235(B)] (ab175240) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : HeLa lysate
      Lane 3 : Jurkat lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 47 kDa

    • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling PUS1 with ab175240 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling PUS1 with ab175240 at 1/50 dilution.

    • Flow Cytometrical analysis of permeabilized Jurkat cells labeling PUS1 with ab175240 antibody at 1/10 (red) or a Rabbit IgG (negative) (green).

    References

    ab175240 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175240.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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