Key features and details
- Rabbit polyclonal to PUS10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PUS10 antibody
See all PUS10 primary antibodies
DescriptionRabbit polyclonal to PUS10
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human stomach tissue; WB: Transfected HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab184981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.|
FunctionPseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
Sequence similaritiesBelongs to the pseudouridine synthase Pus10 family.
modificationsProteolytically cleaved during TRAIL-induced cell death. Cleaved, in vitro, either by caspase-3 or caspase-8.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CCDC139 antibody
- Coiled coil domain containing 139 antibody
- Coiled coil domain containing protein 139 antibody
Immunohistochemical analysis of human stomach tissue labeling PUS10 at the cytoplasm and membrane in glandular cells with ab184981 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-PUS10 antibody (ab184981) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PUS10 over-expressed into HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 60 kDa
ab184981 has not yet been referenced specifically in any publications.