Key features and details
- Rabbit polyclonal to PUS10
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PUS10 antibody
See all PUS10 primary antibodies
DescriptionRabbit polyclonal to PUS10
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: RT4 whole cell lysate; IHC-P: Human stomach tissue; ICC: A431 whole cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
Sequence similaritiesBelongs to the pseudouridine synthase Pus10 family.
modificationsProteolytically cleaved during TRAIL-induced cell death. Cleaved, in vitro, either by caspase-3 or caspase-8.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CCDC139 antibody
- Coiled coil domain containing 139 antibody
- Coiled coil domain containing protein 139 antibody
Anti-PUS10 antibody (ab185078) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate
Predicted band size: 60 kDa
Immunohistochemistry analysis of human stomach tissue labeling PUS10 cytoplasm and nucleus in glandular cells with ab185078 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry analysis of A431 (human epidermoid carcinoma cell line) whole cells labeling PUS10 nucleoplasm, nuclear bodies and mitochondria with ab185078 at 2 µg/ml.
ab185078 has not yet been referenced specifically in any publications.