Overview

  • Product name

    Anti-PUS3 antibody - C-terminal
  • Description

    Rabbit polyclonal to PUS3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cat, Pig, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PUS3 aa 431-481. The exact sequence is proprietary.
    Sequence:

    VRRGRIEHPH LFHEEETKAK RDCNDTLEEE NTNLETPTKR VCVDTEIKSII


    Database link: Q9BZE2

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab211270 was affinity purified using an epitope specific to PUS3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-PUS3 antibody - C-terminal (ab211270) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 30 seconds
  • Detection of PUS3 in Immunoprecipitates of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using: 1) ab211270 at 6 µg per reaction. 2) control IgG. For blotting ab211270 was used at 1 µg/ml. Chemiluminescence with an exposure time of 30 seconds.

References

ab211270 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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