Key features and details
- Rabbit polyclonal to PUS3 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PUS3 antibody - C-terminal
DescriptionRabbit polyclonal to PUS3 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cat, Pig, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab211270 was affinity purified using an epitope specific to PUS3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab211270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionFormation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.
Sequence similaritiesBelongs to the tRNA pseudouridine synthase truA family.
- Information by UniProt
- 2610020J05Rik antibody
- FKSG32 antibody
- pseudouridylate synthase 3 antibody
All lanes : Anti-PUS3 antibody - C-terminal (ab211270) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30 seconds
Detection of PUS3 in Immunoprecipitates of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using: 1) ab211270 at 6 µg per reaction. 2) control IgG. For blotting ab211270 was used at 1 µg/ml. Chemiluminescence with an exposure time of 30 seconds.
ab211270 has not yet been referenced specifically in any publications.