Anti-PUS7 antibody (ab224119)
Key features and details
- Rabbit polyclonal to PUS7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PUS7 antibody
See all PUS7 primary antibodies -
Description
Rabbit polyclonal to PUS7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human PUS7 aa 500 to the C-terminus.
Database link: Q96PZ0 -
Positive control
- WB: K562, NIH/3T3 and NBT-II cell lysates. IHC-P: Human testis, pancreas, cervix and endometrium tissue. ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 75 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 75 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Sequence similarities
Belongs to the pseudouridine synthase TruD family.
Contains 1 TRUD domain. - Information by UniProt
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Database links
- Entrez Gene: 615644 Cow
- Entrez Gene: 54517 Human
- Entrez Gene: 78697 Mouse
- Entrez Gene: 296751 Rat
- Omim: 616261 Human
- SwissProt: Q08DI8 Cow
- SwissProt: Q96PZ0 Human
- Unigene: 520619 Human
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Alternative names
- FLJ20485 antibody
- KIAA1897 antibody
- MGC17720 antibody
see all
Images
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Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human endometrium sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-PUS7 antibody (ab224119) at 0.4 µg/ml + K562 cell lysates
Predicted band size: 75 kDa -
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PUS7 (green) using ab224119 (4 µg/ml) in ICC/IF.
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All lanes : Anti-PUS7 antibody (ab224119) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 75 kDa -
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human testis sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human cervix uterine sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab224119 has been referenced in 2 publications.
- Song D et al. HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance. J Exp Clin Cancer Res 40:170 (2021). PubMed: 33990203
- Du J et al. Pseudouridylate Synthase 7 Promotes Cell Proliferation and Invasion in Colon Cancer Through Activating PI3K/AKT/mTOR Signaling Pathway. Dig Dis Sci N/A:N/A (2021). PubMed: 33811565