Overview

  • Product name

  • Description

    Rabbit polyclonal to PUS7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human PUS7 aa 532-654.
    Sequence:

    PGFDVIYPKHKIQEAYREMLTADNLDIDNMRHKIRDYSLSGAYRKIIIRP QNVSWEVVAYDDPKIPLFNTDVDNLEGKTPPVFASEGKYRALKMDFSLPP STYATMAIREVLKMDTSIKNQTQ


    Database link: Q96PZ0

  • Positive control

    • WB: RT4, U251 MG sp, NIH/3T3 and NBT-II cell lysates. IHC-P: Human testis tissue. ICC/IF: U-251 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 75 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/permeabilization: PFA/Triton X-100

Target

Images

  • Paraffin-embedded human testis tissue stained for PUS7 with ab224119 (1/200) in immunohistochemical analysis

  • All lanes : Anti-PUS7 antibody (ab224119) at 1/100 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate

    Predicted band size: 75 kDa

  • All lanes : Anti-PUS7 antibody (ab224119) at 1/100 dilution

    Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 2 : NBT-II (Rat Wistar bladder tumor cell line) cell lysate

    Predicted band size: 75 kDa

  • PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PUS7 (green) using ab224119 (4 µg/ml) in ICC/IF.

References

ab224119 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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