Anti-PUS7 antibody (ab226257)
Key features and details
- Rabbit polyclonal to PUS7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PUS7 antibody
See all PUS7 primary antibodies -
Description
Rabbit polyclonal to PUS7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human PUS7 aa 100-150. The exact sequence is proprietary.
Database link: Q96PZ0 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226257 was affinity purified using an epitope specific to PUS7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/25000. Predicted molecular weight: 75 kDa.
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| Notes |
|---|
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WB
1/10000 - 1/25000. Predicted molecular weight: 75 kDa. |
Target
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Sequence similarities
Belongs to the pseudouridine synthase TruD family.
Contains 1 TRUD domain. - Information by UniProt
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Database links
- Entrez Gene: 472481 Chimpanzee
- Entrez Gene: 102137970 Cynomolgus monkey
- Entrez Gene: 101151571 Gorilla
- Entrez Gene: 54517 Human
- Entrez Gene: 100173954 Orangutan
- Entrez Gene: 697463 Rhesus monkey
- Omim: 616261 Human
- SwissProt: Q96PZ0 Human
see all -
Alternative names
- FLJ20485 antibody
- KIAA1897 antibody
- MGC17720 antibody
see all
Images
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Western blot - Anti-PUS7 antibody (ab226257)All lanes : Anti-PUS7 antibody (ab226257) at 0.04 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab226257 has not yet been referenced specifically in any publications.
